Cell and Organ Transplantology. 2017; 5(1):14-22.
DOI: 10.22494/COT.V5I1.65
The effect of human adipose-derived multipotent mesenchymal stromal cells in the fibrin gel on the healing of full-thickness skin excision wounds in mice
Tykhvynskaya O. A., Rogulska O. Yu., Volkova N. A., Revenko E. B., Mazur S. P., Volina V. V., Grischuk V. P., Petrenko A. Yu., Petrenko Yu. A.
Institute for Problems of Cryobiology and Cryomedicine of National Academy of Science of Ukraine, Kharkiv, Ukraine
Abstract
Prospects for the widespread use of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine determine the relevance of studying their abilities to affect the reparative process in experimental systems in vivo.
Materials and methods. The effect of human adipose-derived MSCs on the healing rate and completeness of damaged skin site reconstitution was examined using full-thickness excision wound model in mice. The reparative activity of MSCs was revealed in planimetric and histological studies. Human blood plasma-derived fibrin gel was used as a scaffold for MSCs delivery.
Results and conclusions. Compared to the spontaneous healing process, application of fibrin gel on the excisional skin wounds promotes earlier maturation of granulation tissue and further formation of loose scar tissue with skin derivates. MSCs in the fibrin gel contribute to the improve of wound epithelialization, the decrease of the inflammatory response, faster maturation of the granulation tissue, including marks of angiogenesis, as well as promotes complete recovery of the dermal and epidermal layers of the damaged site of skin.
Key words: adipose-derived multipotent mesenchymal stromal cells; fibrin gel; excisional skin wounds; wound healing
Full Text PDF (eng) Full Text PDF (ua)1. Borena BM, Martens A, Broeckx SY, et al. Regenerative Skin Wound Healing in Mammals: State–of–the–Art on Growth Factor and Stem Cell Based Treatments. Cell Physiol Biochem. 2015; 36(1): 1-23. https://doi.org/10.1159/000374049 PMid:25924569 |
||||
2. Jackson WM, Nesti LJ, Tuan RS. Mesenchymal stem cell therapy for attenuation of scar formation during wound healing. Stem Cell Research & Therapy. 2012; 3(20): 1-9. https://doi.org/10.1186/scrt111 |
||||
3. Rustad KC, Wong VW, Sorkin M, et al. Biomaterials enhancement of mesenchymal stem cell angiogenic capacity and stemness by a biomimetic hydrogel scaffold. Biomaterials. 2012; 33(1): 80-90. https://doi.org/10.1016/j.biomaterials.2011.09.041 PMid:21963148 PMCid:PMC3997302 |
||||
4. Atkinson K. The biology and therapeutic application of mesenchymal cells. New Jersey: John Wiley & Sons, 2017. 1007 p. | ||||
5. Wu Y, Chen L, Scott PG, et al. Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis. Stem Cells. 2007; 25(10): 2648-59. https://doi.org/10.1634/stemcells.2007-0226 PMid:17615264 |
||||
6. Rodriguez J, Boucher F, Lequeux C, et al. Intradermal injection of human adipose – derived stem cells accelerates skin wound healing in nude mice. Stem Cell Research & Therapy. 2015; 6(241): 1-11. https://doi.org/10.1186/s13287-015-0238-3 |
||||
7. Duscher D, Barrera J, Wong VW, et al. Stem cells in wound healing: the future of regenerative medicine? A mini–review. Gerontology. 2015; 62(2): 216-25. https://doi.org/10.1159/000381877 PMid:26045256 |
||||
8. Clark RAF, Ghosh K, Tonnesen MG. Tissue engineering for cutaneous wounds. Journal of Investigative Dermatology. 2007; 127(5): 1018-29. https://doi.org/10.1038/sj.jid.5700715 PMid:17435787 |
||||
9. Yudintseva NM, Pleskach NM, Smagina LV, et al. Vosstanovlenie soedinitel’noy tkani v rezul’tate transplantatsii na rany eksperimental’nykh zhivotnykh dermal’nogo ekvivalenta na osnove fibrina [Reconstruction of Connective Tissue from Fibrin-Based Dermal Equivalent Transplanted to Animals with Experimental Wounds]. Tsitologiya – Cytology. 2010; 52(9): 724-28. [In Russian] | ||||
10. Bensaid W, Triffitt JT, Blanchat C, et al. A biodegradeable fibrin scaffold for mesenchymal stem cell transplantation. Biomaterials. 2003; 24: 2497-502. https://doi.org/10.1016/S0142-9612(02)00618-X |
||||
11. Sánchez M, Anitua E, Orive G, et al. Platelet–Rich Therapies in the Treatment of Orthopaedic Sport Injuries. Sports Medicine. 2009; 39(5): 345-54. https://doi.org/10.2165/00007256-200939050-00002 PMid:19402740 |
||||
12. Blanton MW, Hadad I, Johnstone BH, et al. Adipose stromal cells and platelet–rich plasma therapies synergistically increase revascularization during wound healing. Plastic and Reconstructive Surgery. 2009; 123 (suppl): 56S-64S. https://doi.org/10.1097/PRS.0b013e318191be2d PMid:19182664 |
||||
14. Petrenko YA,. Petrenko AY. Phenotypical properties and ability to multilineage differentiation of adipose tissue stromal cells during subculturing. Cytol Genet. 2012; 46: 36-40. https://doi.org/10.3103/S0095452712010070 |
||||
15. Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue engineering. 2001; 7(2): 211-28. https://doi.org/10.1089/107632701300062859 PMid:11304456 |
||||
16. Obata S, Akeda K, Imanishi T, et al. Effect of autologous platelet–rich plasma–releasate on intervertebral disc degeneration in the rabbit anular puncture model: a preclinical study. Arthritis Research & Therapy. 2012; 14(6): 1-11. https://doi.org/10.1186/ar4084 PMid:23127251 PMCid:PMC3674597 |
||||
17. Galiano RD, Michaels JV, Dobryansky M, et al. Quantitative and reproducible murine model of excisional wound healing. Wound Repair and Regeneration. 2004; 12(4): 485-92. https://doi.org/10.1111/j.1067-1927.2004.12404.x PMid:15260814 |
||||
18. Hammer Ø, Harper DAT, Ryan PD. Paleontological statistics software package for education and data analysis. Palaeontol Electron. 2001; 4: 9-18. | ||||
19. Liu X, Wang Z, Wang R, et al. Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds. Int J Mol Med. 2013; 31: 407-15. PMid:23228965 |
||||
20. Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy. 2006; 8(4): 315-17. https://doi.org/10.1080/14653240600855905 PMid:16923606 |
||||
21. Yang Y, Zhang W, Li Y, et al. Scalded skin of rat treated by using fibrin glue combined with allogeneic bone marrow mesenchymal stem cells. Annals of Dermatology. 2014; 26(3): 289-95. https://doi.org/10.5021/ad.2014.26.3.289 PMid:24966626 PMCid:PMC4069637 |
||||
22. Falanga V, Iwamoto S, Chartier M, et al. Autologous bone marrow–derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds. Tissue Engineering. 2007; 13(6): 1299-312. https://doi.org/10.1089/ten.2006.0278 PMid:17518741 |
||||
23. Mansilla E, Marin GH, Sturla F, et al. Human mesenchymal stem cells are tolerized by mice and improve skin and spinal cord injuries. Transplantation Proceedings. 2005; 37(1): 292-94. https://doi.org/10.1016/j.transproceed.2005.01.070 PMid:15808623 |
||||
24. Isakson M, de Blacam C, Whelan D, et al. Mesenchymal stem cells and cutaneous wound healing: current evidence and future potential. Stem Cells International. 2015; 2015: 1-12. https://doi.org/10.1155/2015/831095 PMid:26106431 PMCid:PMC4461792 |
||||
25. Mehanna RA, Nabil I, Attia N, et al. The effect of bone marrow–derived mesenchymal stem cells and their conditioned media topically delivered in fibrin glue on chronic wound healing in rats. BioMed Research International. 2015; 2015: 1-12. https://doi.org/10.1155/2015/846062 PMid:26236740 PMCid:PMC4508387 |
Tykhvynskaya OA, Rogulska OYu, Volkova NA, Revenko EB, Mazur SP, Volina VV, Grischuk VP, Petrenko AYu, Petrenko YuA. The effect of human adipose-derived multipotent mesenchymal stromal cells in the fibrin gel on the healing of full-thickness skin excision wounds in mice. Cell and Organ Transplantology. 2017; 5(1):14-22. doi:10.22494/cot.v5i1.65
Is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.