Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Home/2015, Vol. 3, No. 1/Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Cell and Organ Transplantology. 2015; 3(1):34-38.
DOI: 10.22494/COT.V3I1.18

Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Prokopyuk V. Yu., Prokopyuk O. S., Musatova I. B., Shevchenko N. А., Roenko A. А., Terehova E. A., Volina V. V.
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

There have been studied morphological safety and functional state of the explants of human placenta, umbilical cord and fetal membranes by vital staining techniques using the MTT and resazurin reduction tests, level of glucose in incubation medium, activity of lactate dehydrogenase and alkaline phosphatase before and after cryopreservation. It has been found that proposed program of cryopreservation allows keeping a high level of viability of the explants of placenta, umbilical cord and fetal membranes, the most informative methods of assessing the safety of these biological objects before and after cryopreservation are method of vital staining, determination of glucose content in incubation medium, MTT-test and resazurin reduction test.

Key words: placenta; umbilical cord; fetal membrane explants; cryopreservation

Full Text PDF (eng) Full Text PDF (ru)

1. Korzhevsky DE, Merkulov AV. Osnovy gistologicheskoj tehniki [Fundamentals of histological techniques]. SPb.: SpecLit – SPb.: SpecLit. 2010; 95 p. In Russian.
2. Musatova IB, Prokopyuk OS, Volin VV, et al. Sozdanie kriozashhitnyh sred dlja sohranenija jeksplantov tkani placenty [Creating of cryoprotective media for storing placental tissue explants]. Biotechnologia acta – Biotechnology. 2013; 6(6):132−138. In Russian.
3. Prokopyuk OS, Petrenko AYu, Prokopyuk VYu, et al. Kriokonservirovanie placenty razlichnoj stepeni zrelosti [Cryopreservation of placenta with different maturity degree]. Problemy kriobiologii – Problems of Cryobiology. 2008; 18(2):220. In Russian.
4. Shablii V, Kuchma M, Kyryk V, et al. Mezinhimal’nye i stromal’nye kletki iz nativnoj i kriokonservirovannoj placenty cheloveka: fenotip, mul’tipotentnost’ i migracionnyj potencial in vivo. [Mesenchymal and stromal cells of native and cryopreserved human placenta: phenotype, multipotent and migration potential in vivo]. Problemy kriobiologii − Problems of Cryobiology. 2012; 22(2):157 − 160.
5. Shablii V, Kuchma M, Kyryk V, et al. Fenotypova harakterystyka kul’tury klityn, oderzhanoi’ iz kriokonservovanoi’ tkanyny horiona [Phenotypic characteristics of cell cultures derived from cryopreserved chorion tissue]. Biotehnologija – Biotechnology. 2012; 5(2):76-85.
6. Allen CL, Clare G, Stewart EA, et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One. 2013; 30(8):1−15.
7. Colleoni F, Morash AJ, Ashmore T, et al. Cryopreservation of placental biopsies for mitochondrial respiratory analysis. Placenta. 2012; 33(2):122−123.
8. Cooke M, Tan EK, Mandrycky C, et al. Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated amniotic membrane/chorion tissue. J Wound Care. 2014; 23(10):465−476.
9. Garrod A, Batra G, Ptacek I, et al. Duration and method of tissue storage alters placental morphology – implications for clinical and research practice. Placenta. 2013; 34(11):1116−1119.
10. Huppertz B, Kivity V, Sammar M, et al. Cryogenic and low temperature preservation of human placental villous explants. A new way to explore drugs in pregnancy disorders. Placenta. 2011; 32:65−76.
11. Kesting MR, Loeffelbein DJ, Steinstraesser L, et al. Cryopreserved human amniotic membrane for soft tissue repair in rats. Ann Plast Surg. 2008; 60(6):684−691.
12. Laurent R, Nallet A, Obert L, et al. Storage and qualification of viable intact human amniotic graft and technology transfer to a tissue bank. Cell Tissue Bank. 2014; 15(2):267−275.
13. Madhavan HN, Priya K, Malathi J, et al. Preparation of amniotic membrane for ocular surface reconstruction. Indian J Ophthalmol. 2002; 50(3):227−231.
14. Martins JP, Santos JM, de Almeida JM, et al. Towards an advanced therapy medicinal product based on mesenchymal stromal cells isolated from the umbilical cord tissue: quality and safety data. Stem Cell Res Ther. 2014; 5(19):1−15.
15. Niknejad H, Deihim T, Solati-Hashjin M, et al. The effects of preservation procedures on amniotic membrane’s ability to serve as a substrate for cultivation of endothelial cells. Cryobiology. 2011; 63(3):145−151.
16. Okabe M, Kitagawa K, Yoshida T, et al. Hyperdry human amniotic membrane is useful material for tissue engineering: physical, morphological properties, and safety as the new biological material. J Biomed Mater Res A. 2014; 102(3):862−870.
17. Pipino C, Shangaris P, Resca E, et al. Placenta as a reservoir of stem cells: an underutilized resource. Br Med Bull. 2013; 105:43−68.
18. Ravishanker R, Bath AS, Roy R. “Amnion Bank”- the use of long term glycerol preserved amniotic membranes in the management of superficial and superficial partial thickness burns. Burns. 2003; 29(4):369−374.
19. Roselli EA, Lazzati S, Iseppon F, et al. Fetal mesenchymal stromal cells from cryopreserved human chorionic villi: cytogenetic and molecular analysis of genome stability in long-term cultures. Cytotherapy. 2013; 15(11):1340−1351.
20. Taghizadeh RR, Cetrulo KJ, Cetrulo CL. Wharton’s Jelly stem cells: future clinical applications. Placenta. 2011; 2(4):311−315.
21. Thomasen H, Pauklin M, Noelle B, et al. The effect of long-term storage on the biological and histological properties of cryopreserved amniotic membrane. Curr Eye Res. 2011; 36(3):247−255.

Prokopyuk VYu, Prokopyuk OS, Musatova IB., Shevchenko NА, Roenko AА, Terehova EA, Volina VV. Safety of placental, umbilical cord and fetal membrane explants after cryopreservation. Cell and Organ Transplantology. 2015; 3(1):34-38. doi: 10.22494/COT.V3I1.18


Creative Commons License
Is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.