Effect of transplantation of cell suspension from embryonic nervous tissue and bone marrow on postischemic cerebral angiogenesis and restoration of limb motor function in rats with experimental ischemic stroke

Cell and Organ Transplantology. 2015; 3(2):125-132.
DOI: 10.22494/COT.V3I2.16

Effect of transplantation of cell suspension from embryonic nervous tissue and bone marrow on postischemic cerebral angiogenesis and restoration of limb motor function in rats with experimental ischemic stroke

Iarmoliuk Ie. S.^1,2, Tsymbaliuk V. І.^1,2, Staino L. P.², Savchuk О. V.³, Diatel М. V.⁴¹Bogomolets National Medical University, Kyiv, Ukraine
²A. P. Romodanov State Institute of Neurosurgery NAMS of Ukraine, Kyiv, Ukraine
³Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv, Ukraine
⁴Kyiv City Oncology Center, Kyiv, Ukraine

Absctract
Ischemic stroke is one of the leading causes of mortality and disability worldwide. Dispite the progress of medical knowledge and technologies, the rate of permanent neurological impairment in patients after stroke remains high and effective strategy of restorative treatment is still at the stage of experimental development. Restoration of nervous system functions after stroke implies the activation of endogenous reparative processes, such as angiogenesis, using sources of regenerative medicine, including cell and tissue transplantation. Development of optimal and safe methods of neurotransplantation for stroke is one of the priorities of experimental research in this field.
Purpose: to study the effect of post-stroke angiogenesis, stimulated by transplantation of cell suspension from embryonic nervous tissue (TCS-ENT) and bone marrow (TCS-BM), on restoration of motor functions in rats with experimental stroke.
Materials and methods. 160 adult (3-4 months old) outbred albino rats weighing between 280-320 g were divided into groups and subgroups depending on the experimental procedure: with isolated middle cerebral artery occlusion (MCAO), intracerebral allotransplantation of cell suspension from embryonic nervous tissue (MCAO + TCS-ENT),intracerebral autotransplantation of cell suspension from bone marrow (MCAO + TCS-BM) or phosphate-buffered 0.9 % saline infusion (MCAO + PBS) on the 2^nd day after MCAO. MCAO was conducted using the modified method of intraluminal monofilament occlusion with blocking of collaterals. Volume of infarction zone was estimated using TTC staining on 7^th and 14^th day (n = 5 from each group on each day), number of vessels in periinfarct zone was calculated by immunohistochemical staining for CD34 on 7^th, 14^th and 28^th day (n = 6 from each group on each day) after MCAO. Motor deficit was assessed by ledged tapered beam-walking test on 1^st, 3^rd, 7^th, 14^th, 21^st and 28^th day after MCAO (n = 18 from each group on each day).
Results.TCS-BM caused the increase in the number of vessels in the periinfarct zone in dynamics, most prominent on 28^th day, and decrease in the volume of infarction zone in comparison with other experimental groups, starting on 7^th day after MCAO. TCS-ENT and TCS-BM resulted in regression of motor deficit, starting from 3^rd and till 28^th day after MCAO. The degree of limb motor asymmetry in rats negatively correlated with the number of vessels in periinfarct zone.
Conclusion. Transplantation of cell suspension from embryonic nervous tissue and bone marrow promotes the regression of motor impairments in experimental animals due to angiogenic effect, which is more prominent in case of TCS-BM.

Key words: middle cerebral artery occlusion, embryonic nervous tissue, bone marrow, angiogenesis, motor deficit

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How to Cite this Article

Iarmoliuk IeS, Tsymbaliuk VІ, Staino LP, Savchuk ОV, Diatel МV. Effect of transplantation of cell suspension from embryonic nervous tissue and bone marrow on postischemic cerebral angiogenesis and restoration of limb motor function in rats with experimental ischemic stroke. Cell and Organ Transplantology. 2015; 3(2):125-132. doi: 10.22494/COT.V3I2.16

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