Biocompatibility analysis of the decellularized bovine pericardium

Home/2020, Vol. 8, No. 2/Biocompatibility analysis of the decellularized bovine pericardium

Cell and Organ Transplantology. 2020; 8(2):112-116.
DOI: 10.22494/cot.v8i2.110

Biocompatibility analysis of the decellularized bovine pericardium

Sokol A.1,2, Grekov D.1,2, Yemets G.1, Galkin O.2, Shchotkina N.1,2, Rudenko N.1, Yemets I.1

  • 1Ukrainian Children’s Cardiac Center, Kyiv, Ukraine
  • 2Igor Sikorsky Kyiv Polytechnic Institute, National Technical University of Ukraine, Kyiv, Ukraine

Abstract
The decellularized bovine pericardium and its potential use as a natural scaffold is a promising approach in the field of tissue engineering and regenerative medicine. The reaction of the host toward decellularized scaffolds depends on their biocompatibility, which should be satisfied being before applied in clinical use.
Purpose: to evaluate the biocompatibility of the extracellular matrices, which were decellularized by trypsin enzyme and anionic sodium dodecyl sulfate (SDS) detergent.
Material and methods. Pericardial sacs were acquired from 12-18 months’ age bulls. Tissue decellularization was performed by using 0.25 % Trypsin solution and 1 % ionic SDS for group I and 0.1 % SDS for group II samples. The implantation was performed on Wistar rats. The tissue samples were stained with hematoxylin & eosin, Congo red and Masson’s Trichrome for histological analysis.
Results. In group 1 in 3 months after subcutaneous implantation in rats we noticed the inflammation in surrounding tissue and degradation of the implant. Under the same conditions in animals of group 2 implant replacement with growing immature connective tissue was noted. Bio-implant of this group did not degrade, moreover it’s integrated to the tissues of experimental rats.
Conclusion. Our results showed that decellularized bovine pericardium by 0.1 % SDS can become an alternative material for tissue engineering and has the potential for further use in human surgery.

Key words: bovine pericardium; decellularization; implantation; regenerative medicine 

Full Text PDF (preprint)

1. Nordmeyer S, Murin P, Schulz A, et al: Results of aortic valve repair using decellularized bovine pericardium in congenital surgery. Eur J Cardiothorac Surg. 2018; 54:986-992. DOI:10.1093/ejcts/ezy181.
https://doi.org/10.1093/ejcts/ezy181
PMid:29718178
2. Bell D, Prabhu S, Betts K, et al. Durability of tissue-engineered bovine pericardium (CardioCel®) for a minimum of 24 months when used for the repair of congenital heart defects. Interact Cardiovasc Thorac Surg. 2019; 28(2):284-290. DOI: 10.1093/icvts/ivy246.
https://doi.org/10.1093/icvts/ivy246
PMid:30101317
3. Biasi GM, Sternjakob S, Mingazzini PM, Ferrari SA. Nine-year experience of bovine pericardium patch angio-plasty during carotid endarterectomy. J Vasc Surg. 2002; 36:271-277. DOI:10.1067/mva.2002.123685.
https://doi.org/10.1067/mva.2002.123685
PMid:12170207
4. Hoffman J I E, Kaplan S. The incidence of congenital heart disease. J Am Coll Cardiol. 2002; 39(12):1890-900. DOI: 10.1016/s0735-1097(02)01886-7.
https://doi.org/10.1016/S0735-1097(02)01886-7
5. Cunanan CM, Cubbling CM, Dinh TT, et al. Rutledge 3rd and M.C. Fishbein, Tissue characterization and calcification potential of commercial bioprosthetic heart valve. Ann Thora. Surg. 2001; 71:417-421. DOI:10.1016/S0003-4975(01)02493-6.
https://doi.org/10.1016/S0003-4975(01)02493-6
6. Karpenko AA, Kuzhuget RA, Starodubtsev VB, et al. Immediate and long-term results of various methods of carotid bifurcation reconstruction. Circulatory pathology and cardiac surgery. 2013; 1:21-24.
7. Mueller C, Dave H, Prêtre R. Surgical repair of aorto-ventricular tunnel. Multimed Man Cardiothorac Surg. 2012: 1093-7.
https://doi.org/10.1093/mmcts/mms006
PMid:24414710
8. Chesnov UM. Biocompatibility of xenopericardium fixed with epoxy compounds in in vitro and in vivo experiments. Current issues of cardiology: Sat. scientific tr. Manak NA, editor. Minsk: Encyclopedia. 2002. 2:188-90.
9. Nonaka M, Iwakura A, Yamanaka K. Technique to treat extensive abscesses in double valve replacement for prosthetic valve endocarditis. J Heart Valve Dis. 2013; 22 (4):575-7.
https://doi.org/10.7243/2052-5958-1-4
10. Akhmedov SD, Afanasyev SA, Dyakova ML, et al. Use of a cell-free matrix for the formation of new blood vessels and heart by tissue engineering. Cell transplantology and tissue engineering. 2009; 4 (2):32-9.
11. Salameh A, Greimann W, Vondrys D, Kostelka M. Calcification or Not. This Is the Question. A 1-Year Study of Bovine Pericardial Vascular Patches (CardioCel) in Minipigs Semin Thorac Cardiovasc Surg. 2018; 30(1):54-59. DOI: 10.1053/j.semtcvs.2017.09.013.
https://doi.org/10.1053/j.semtcvs.2017.09.013
PMid:29024719
12. Park S, Kim SH, Lim HG, et al. The Anti-calcification Effect of Dithiobispropionimidate, Carbodiimide and Ultraviolet Irradiation Cross-linking Compared to Glutaraldehyde in Rabbit Implantation Models. Korean J Thorac Cardiovasc Surg. 2013; 46(1):1-13. DOI: 10.5090/kjtcs.2013.46.1.1.
https://doi.org/10.5090/kjtcs.2013.46.1.1
PMid:23424053 PMCid:PMC3573159
13. Simões IN, Vale P, Soker S, et al. Acellular Urethra Bioscaffold: Decellularization of Whole Urethras for Tissue Engineering Applications. Sci Rep. 2017; 7:41934. DOI:10.1038/srep41934.
https://doi.org/10.1038/srep41934
PMid:28165009 PMCid:PMC5292742
14. Gilbert WT, Sellaro LT, Badylak FS. Decellularization of tissues and organs. Biomaterials. 2006; 27:3675-3683. DOI: 10.1016/j.biomaterials.2006.02.014.
https://doi.org/10.1016/j.biomaterials.2006.02.014
15. Aamodt JM, Grainger DW. Extracellular matrix-based biomaterial scaffolds and the host response. Biomaterials 2016; 86:68-82. DOI: 10.1016/j.biomaterials.2016.02.003.
https://doi.org/10.1016/j.biomaterials.2016.02.003
PMid:26890039 PMCid:PMC4785021
16. Oswal D, Korossis S, Mirsadraee S, et al. Biomechanical characterization of decellularized and cross-liked bovine pericardium. J Heart Valve Dis. 2007; 16:165-174.
17. Courtman D, Pereira C, Kashef V, McComb D. Development of a pericardial acellular matrix biomaterial: Biomechanical and mechanical effects of cell extraction. J Biomed Mater Res. 1994; 28: 655-666. DOI:10.1002/jbm.820280602.
https://doi.org/10.1002/jbm.820280602
PMid:8071376
18. Luo J, Korossis S, Wilshaw SP, et al. Development and characterization of acellular porcine pulmonary valve scaffolds for tissue engineering. Tissue Eng. A. 2014; 20:2963-2974. DOI:10.1089/ten.tea.2013.0573.
https://doi.org/10.1089/ten.tea.2013.0573
PMid:24786313 PMCid:PMC4229718
19. Korossis S, Wilcox H, Watterson K., et al. In-vitro assessment of the functional perfor-mance of the decellularized intact porcine aortic root. J Heart Valve Dis. 2005; 14:408-422.
20. Yeh HS, Keller JT, Brackett KA, et al. Human umbilical artery for microvascular grafting.Experimental study in the rat. J Neurosurg. 1984; 61:737.
https://doi.org/10.3171/jns.1984.61.4.0737
PMid:6470785
21. Schmidt CE, Baier JM. Acellular vascular tissues: Natural biomaterials for tissue repair and tissue engineering. Biomaterials. 2000; 21:2215. DOI:10.1016/S0142-9612(00)00148.
https://doi.org/10.1016/S0142-9612(00)00148-4
22. Andre’e B, Bela K, Horvath T, et al. Successful re-endothelialization of a perfusable biological vascularized matrix (BioVaM) for the generation of 3D artificial cardiac tissue. Basic Res Cardiol. 2014; 109:441.
https://doi.org/10.1007/s00395-014-0441-x
PMid:25231595
23. Ning Lia, Yang Lia, Dejun Gong, et al: Efficient decellularization for bovine pericardium with extracellular matrix preservation and good biocompatibility. ICVTS. 2018; 26:68-776. DOI:10.1093/icvts/ivx416.
https://doi.org/10.1093/icvts/ivx416
PMid:29340634
24. Tran HLB, Dihn TH, Nguyen TN, et al. Preparation and characterization of acellular porcine pericardium for cardiovascular surgery. Turk J Biol. 2016; 40:1243-1250. DOI:10.3906/biy-1510-44.
https://doi.org/10.3906/biy-1510-44

Sokol A, Grekov D, Yemets G, Galkin O, Shchotkina N, Rudenko N, Yemets I. Biocompatibility analysis of the decellularized bovine pericardium. Cell and Organ Transplantology. 2020; 8(2):112-116. doi:10.22494/cot.v8i2.110

Creative Commons License
Is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.