Comparative morphometric study of adipose tissue in ovariectomized mice of different ages

Home/2020, Vol. 8, No. 1/Comparative morphometric study of adipose tissue in ovariectomized mice of different ages

Cell and Organ Transplantology. 2020; 8(1):in press.
DOI: 10.22494/cot.v8i1.108

Comparative morphometric study of adipose tissue in ovariectomized mice of different ages

Ivanishchev V.1, Ustymenko A.1,2, Kyryk V.1,2, Butenko G.1,2

  • 1Chebotarev State Institute of Gerontology of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • 2State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine


It is known that estrogen deficiency leads to the dysfunction of many organs and systems, including adipose tissue. Therefore, the study of morphological changes in the components of adipose tissue during estrogen deficiency will expand our understanding of both its dysfunction and possible ways to correct it.
The purpose of the study is to conduct a comparative analysis of morphometric parameters of subcutaneous adipose tissue in ovariectomized mice of different ages.
Materials and methods. Subcutaneous adipose tissue samples from young (2 months) and old (14 months) mice were studied two months after ovariectomy in comparison with the samples from sham-operated animals of the same age. The number of adipocytes and capillaries per 1 mm2 of the area and the average area of adipocyte were determined on histological slices of adipose tissue.
Results and discussion. It was shown that along with a significant decrease in the number of adipocytes per 1 mm2 of the area in young ovariectomized mice, a significant increase in the average area of adipocytes was observed compared with the control group of animals of the same age. In old ovariectomized animals at a slight decrease in the number of adipocytes, there was also a probable increase in their average area compared with the control group of the same age.
Conclusion. The morphological properties of subcutaneous adipose tissue change significantly in the conditions of artificially induced estrogen deficiency during ovariectomy in mice.

Key words: subcutaneous adipose tissue; adipocyte; ovariectomy; morphometry


1. Volkova ОV, Yeletsky YuК. Osnovy gistologii s gistologicheskoy tekhnikoy. [Basics of Histology with Histological Technique]. Moskva: Meditsina, 1982. 304 p. [In Russian]
2. Grigoryan OR, Andreeva EN. Sostoyanie uglevodnogo obmena u zhenshchin v period menopauzy [The state of carbohydrate metabolism in women during menopause]. Reproduktivnoe zdorov’e i sakharnyy diabet – Reproductive health and diabetes. 2009; 4:15-20. [In Russian]
3. Dedov II. Zhirovaya tkan’ kak endokrinnyy organ [Adipose tissue as an endocrine organ]. Ozhirenie i metabolizm – Obesity and metabolism. 2006; 1:6-13. [In Russian]
4. Kishkun АА. Biologicheskiy vozrast i starenie: vozmozhnosti opredeleniya i puti korrektsii. Ruk-vo dlya vrachey. [Biological age and aging: possibilities of definition and ways of correction. Manual for doctors]. Moskva: GEOTAR-Media, 2008. 973 p. [In Russian]
5. Myadelets OD. Belaya i buraya zhirovye tkani: vzaimodeystvie so skeletnoy myshechnoy tkan’yu [White and brown adipose tissue: interactions with skeletal muscle tissue]. Vestnik VMGU – VMGU Bulletin. 2014; 13(5):32-44. [In Russian]
6. Ovsyannikova TV, Kulikov IA. Printsipy naznacheniya monoterapii estrogenami. [Principles of prescribing estrogen monotherapy]. Meditsinskiy sovet – Medical advice. 2015; 9:66-70. [In Russian]
7. Serov VN, Prilepskaya VN, Ovsyannikova TV. Ginekologicheskaya endokrinologiya [Gynecological endocrinology]. Moskva: MEDpress, 2015. 528 р. [In Russian]
8. Sulaeva ON, Belemets NI. Polovye osobennosti regulyatsii zhirovoy tkani [Sexual features of adipose tissue regulation]. Klinicheskaya endokrinologiya i endokrinnaya khirurgiya – Clinical endocrinology and endocrine surgery. 2017; 4(60):11-20. [In Russian]
9. Startseva OI, Melnikov DV, Zakharenko AS, Kirillova KA, Ivanov SI, Pischikova ED, et al. Mesenchymal stem cells of adipose tissue: a modern view, the relevance and prospects of application in plastic surgery. Issled. prakt. med. Research’n Practical Medicine Journal. 2016; 3(3):68-75. DOI: 10.17709/2409-2231-2016-3-3-7.
10. Rzepeckiet AK, Murase JE, Juran R, Fabi S, McLellan BN. Estrogen-deficient skin: The role of topical therapy Int J Womens Dermatol. 2019; 5(2):85-90. DOI: 10.1016/j.ijwd.2019.01.001.
PMid:30997378 PMCid:PMC6451761
11. Cao Y. Angiogenesis and vascular function sinmodulation of obesity, adipose metabolism, and insulinsensitivity. Cell Metab. 2013; 18(4):478-89.
12. Campbell SE, Febbraio MA. Effect of the ovarian hormones on GLUT4 expression and contraction-stimulated glucose uptake. Am J Physiol Endocrinol Metab. 2002; 282:1139-1146.
13. Serena С, Keiran N, Ceperuelo-Mallafre V, Ejarque M, Fradera R, Roche K, et al. Obesity and Type 2 Diabetes alters the immune properties of human Adipose Derived Stem Cell. Stem Cell. 2016; 34(10):2559-2573. DOI: 10.1002/stem.2429.
14. Gomes-Gatto CV, Duarte FO, Stotzer US, Rodrigues MF, Perez SE, Selistre de Araujo HS. Estrogen deficiency in ovariectomized rats: can resistance training re-establish angiogenesis in visceral adipose tissue?. Clinics. 2016; 71(9):528-536.
15. Hong J, Stubbins RE, Smith RR, Harvey AE, Núñez NP. Differential susceptibility to obesity between male, female and ovariectomized female mice. 2009; 8(11). DOI: 10.1186/1475-2891-8-11.
PMid:19220919 PMCid:PMC2650703
16. Jo J, Gavrilova O, Pack S, et. al. Hypertrophy and/or Hyperplasia: Dynamics of Adipose Tissue Growth. PLoS Comput Biol. 2009; 5:1000324.
PMid:19325873 PMCid:PMC2653640
17. Leeners B, Geary N, Tobler PN, Asarian L. Ovarian hormones and obesity. Hum Reprod Update. 2017; 23(3):300-321.
PMid:28333235 PMCid:PMC5850121
18. Fatima LA, Campello RS, Santosetal R. Estrogenreceptor 1 (ESR1) regulates VEGFA in adipose tissue. article. Sci Rep. 2017; 7(1). DOI: 10.1038/s41598-017-16686-7.
PMid:29196658 PMCid:PMC5711936
19. Lee H, Choi J, ShikShin S, Yoon M. (Effects of Korean red ginseng (Panaxginseng) on obesity and adipose inflammation in ovariectomized mice. J Ethnopharmacol. 2016; 178:229-237.
20. Levine ME, Lu AT, Chen BH, Hernandez DG, Singleton AB, Ferrucci L, at al. Menopause accelerates biological aging. Proc Natl Acad Sci U S A. 2016; 113(33):9327-9332.
PMid:27457926 PMCid:PMC4995944
21. Nunez NP, Perkins SN, Smith NC, Berrigan D, Berendes DM, Varticovski L, et al. Obesity accelerates mouse mammary tumor growth in the absence of ovarian hormones. Nutr Cancer. 2008; 60(4):534-41.
22. Oliva-Olivera W, Coin-Araguez L, Lhamyani S, Clemente-Postigo M, Torres JA, Bernal-Lopez MR, et al. Adipogenic impairment of adiposetissue-derived mesenchymal stem cells in subjects with metabolic syndrome:possible protective role of FGF2. J Clin Endocrinol Metab. 2017; 102:478-487.
23. Stubbins RE, Najjar К, Holcomb VB, Hong J, Núñez NP. Estrogen alters adipocyte biology and protects female mice from adipocyte inflammation and insulinresistance Diabetes Obes Metab. 2012; 14(1):58-66.
PMid:21834845 PMCid:PMC3236284
24. Rocca WA, Gazzuola-Rocca L, Smith CY, et al. Accelerated Accumulation of Multimorbidity After Bilateral Oophorectomy: A Population-Based Cohort Study. Mayo Clin Proc. 2016; 91(11):1577-1589.
PMid:27693001 PMCid:PMC5097693
25. Shin S, El-Sabbagh AS, Lukas BE, Tanneberger SJ, Jiang, Y. Adipose stem cells in obesity: challenges and opportunities. Bioscience reports. 2020; 40(6). URL:
PMid:32452515 PMCid:PMC7284323
26. Song K, Cai H, Zhang D, et al. Effects of human adipose-derived mesenchymal stem cells combined with estrogen on regulatory T cells in patients with premature ovarian insufficiency. Int Immunopharmacol. 2018; 55:257-262.
27. Zhang Y, Hua F, Ding K, Chen H, Xu Ch, Ding W. Angiogenesis Changes in Ovariectomized Rats with Osteoporosis Treated with Estrogen Replacement Therapy. Bio Res Int. 2019. DOI: 10.1155/2019/1283717.
PMid:31355247 PMCid:PMC6637705
28. Zhang SM, Manson JE, Rexrode KM, Cook NR, Buring JE, Lee IM. Use of oral conjugated estrogen alone and risk of breast cancer. Am J Epidemiol. 2007; 165(5):524-9.

Ivanishchev V, Ustymenko A, Kyryk V, Butenko G. Comparative morphometric study of adipose tissue in ovariectomized mice of different ages. Cell and Organ Transplantology. 2020; 8(1):in press. doi:10.22494/cot.v8i1.108

Creative Commons License
Is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.