The effects of human umbilical cord multipotent mesenchymal stromal cells on the behaviour and oxidative stress in the brain of mice of different ages with a cuprizone-induced model of demyelination

Home/2020, Vol. 8, No. 1/The effects of human umbilical cord multipotent mesenchymal stromal cells on the behaviour and oxidative stress in the brain of mice of different ages with a cuprizone-induced model of demyelination

Cell and Organ Transplantology. 2020; 8(1):in press.
DOI: 10.22494/cot.v8i1.106

The effects of human umbilical cord multipotent mesenchymal stromal cells on the behaviour and oxidative stress in the brain of mice of different ages with a cuprizone-induced model of demyelination

Labunets I.1, Utko N.1, Toporova O.1,2, Panteleymonova T.1, Rodnichenko A.1, Butenko G.1

  • 1State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • 2Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine

Abstract

The transplantation of umbilical cord multipotent mesenchymal stromal cells (UC-MMSCs) is a promising strategy in treatment of multiple sclerosis. The features of cells effect in the recipients of different ages, as well as the ways of its implementation, remain poorly understood.
Purpose. To study the effect of UC-MMSCs transplantation on the behaviour, oxidative stress factors and antioxidant protection in the brain of mice of different ages with an experimental model of multiple sclerosis.
Materials and methods.  Male 129/Sv mice 6-7 months old and 14-16 months old were received neurotoxin cuprizone with food for 3 weeks. Since the 10th day of the cuprizone diet, human UC-MMSCs were injected intravenously at a dose of 5·105 cells per mice. We evaluated the parameters of behaviour in the open field test and rotarod test. The level of malondialdehyde (MDA) and the activity of antioxidant enzymes were studied in the brain.
Results. In mice of both age groups with a cuprizone diet, motor, emotional and exploratory activity decreases. The level of MDA in the brain increases and the activity of antioxidant enzymes decreases. The transplantation of UC-MMSCs leads to positive changes in the behaviour of mice with a cuprizone diet. In the adults, the motor and emotional activity improved; in the aging ones exploratory activity and muscle tone increased. After the injection of UC-MMSCs in adult mice, the level of MDA in the brain decreases.
Conclusion. The therapeutic effect of transplanted UC-MMSCs on the behavioural parameters and MDA level in the brain of mice with a cuprizone model of demyelination largely depends on their age and is more pronounced in adult animals.

Key words: umbilical cord multipotent mesenchymal stromal cells; demyelination; cuprizone; behaviour; oxidative stress

 

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Labunets I, Utko N, Toporova O, Panteleymonova T, Rodnichenko A, Butenko G. The effects of human umbilical cord multipotent mesenchymal stromal cells on the behaviour and oxidative stress in the brain of mice of different ages with a cuprizone-induced model of demyelination. Cell and Organ Transplantology. 2020; 8(1):in press. doi:10.22494/cot.v8i1.106

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