Cell and Organ Transplantology. 2020; 8(1):26-31.
DOI: 10.22494/cot.v8i1.105
Composite chitosan/polyethylene oxide film for duraplasty in traumatic brain injury model in rats
Panteleichuk A.¹, Kadzhaya M.¹, Biloschytsky V.2, Shmeleva A.3, Petriv T.4, Gnatyuk O.5, Dovbeshko G.5, Kozakevych R.6, Tyortyh V.6
- 1Department of neurotrauma, Romodanov State Institute of Neurosurgery of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
- 2Chronic pain treatment group, Romodanov State Institute of Neurosurgery of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
- 3Department of Neuropatomorphology, Romodanov State Institute of Neurosurgery of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
- 4Department of Reconstructive Neurosurgery with X-ray surgery, Romodanov State Institute of Neurosurgery of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
- 5Institute of Physics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
- 6Chuiko Institute of Surface Chemistry of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Abstract
The duraplasty is a standard procedure during neurosurgery for injuries and diseases of the brain. The hermetic closure of the dura mater (DM) not always possible with the application of autologous tissues. Synthetic, allogeneic and xenogenic implants, which are currently used, have disadvantages, so the search for the material that would best meet the requirements for a DM scaffold continues.
The purpose is to study the physical and chemical properties of the composite chitosan/polyethylene oxide (PEO) film and determine the effectiveness of its application for duraplasty in the experiment in vivo; to analyze its ability to biodegradation; to evaluate the effect of chitosan/PEO scaffold on the regeneration of dura matter.
Materials and methods. The experiment used 10 white rats aged 12 months with a penetrating traumatic brain injury model. Postoperative material was examined by macroscopy, optical microscopy and infrared spectroscopy.
Results. According to the analysis of infrared absorption, spectral markers of scar tissue, regenerating DM and intact DM were determined. Oscillation spectroscopy data indicate degradation of the chitosan film and repair of normal DM. Histology data also indicate biological degradation of the chitosan film and its replacement by newly formed normal connective tissue.
Conclusion. The data of morphological and spectroscopic studies show the ability of chitosan/PEO film to biodegradation in vivo with followed replacement not by scar but by normal connective tissue.
Key words: chitosan; polyethylene oxide; penetrating traumatic brain injury; infrared spectroscopy; morphological studies
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MacEwan MR, Kovacs T, Osbun J, Ray Wilson Z Comparative analysis of a fully-synthetic nanofabricated dura substitute and bovine collagen dura substitute in a large animal model of dural repair. Interdisciplinary Neurosurgery. 2018, 13: 145-150. doi: 10.1016/j.inat.2018.05.001.4
https://doi.org/10.1016/j.inat.2018.05.001Matula C, Kjaersgaard L, Di Ieva A. Watertight dural closure in brain surgery: a simple model for training. J Neurol Surg A Cent Eur Neurosurg. 2014; 75(3): 241-5. doi: 10.1055/s-0033-1342928. PubMed PMID: 23681920
https://doi.org/10.1055/s-0033-1342928
PMid:23681920Abuzayed B, Kafadar AM, Oguzoglu SA, Canbaz B, Kaynar MY. Duraplasty using autologous fascia lata reinforced by on-site pedicled muscle flap: technical note. J Craniofac Surg. 2009; 20(2): 435-8. doi: 10.1097/scs.0b013e31819b968f. PubMed PMID: 19326487
https://doi.org/10.1097/SCS.0b013e31819b968f
PMid:19326487Hongtao Sun, Hongda Wang, Yunfeng Diao, Yue Tu, Xiaohong Li, Wanyong Zhao, Jibin Ren, Sai Zhang Large retrospective study of artificial dura substitute in patients with traumatic brain injury undergo decompressive craniectomy Brain Behav. 2018; 8(5): e00907 doi: 10.1002/brb3.907 PMID: 29761002 PMCID: PMC5943738
https://doi.org/10.1002/brb3.907
PMid:29761002 PMCid:PMC5943738Miyake S, Fujita A, Aihara H, Kohmura E. New technique for decompressive duraplasty using expanded polytetrafluoroethylene dura substitute–technical note. Neurol Med Chir (Tokyo). 2006; 46(2):104-6; discussion 106. PMID: 16498223 DOI: 10.2176/nmc.46.104
https://doi.org/10.2176/nmc.46.104
PMid:16498223Schmalz P, Griessenauer C, Ogilvy CS, Thomas AJ. Use of an absorbable synthetic polymer dural substitute for repair of dural defects: a technical note. Cureus. 2018;10 (1):e2127 doi: 10.7759/cureus.2127. PubMed PMID: 29607275; PubMed Central PMCID: PMC5875978
https://doi.org/10.7759/cureus.2127
PMid:29607275 PMCid:PMC5875978Hoell T, Hohaus C, Huschak G, Beier A, Meisel HJ. Total dura substitute in the spine: double layer dural substitute made from polylactide layer and bovine pericardium. Acta Neurochir (Wien). 2007; 149(12):1259-62; doi: 10.1007/s00701-007-1414-8. PMID: 17978882
https://doi.org/10.1007/s00701-007-1414-8
PMid:17978882Narotam PK, Van Dellen JR, Bhoola K, Raidoo D. Experimental evaluation of collagen sponge as a dural graft. Br J Neurosurg. 1993; 7(6): 635-41; doi: 10.3109/02688699308995092. PubMed PMID: 8161425
https://doi.org/10.3109/02688699308995092
PMid:8161425Pettorini BL, Tamburrini G, Massimi L, Paternoster G, Caldarelli M, Di Rocco C. The use of a reconstituted collagen foil dura mater substitute in paediatric neurosurgical procedures – Experience in 47 patients Br J Neurosurg. 2010; 24(1): 51-4. doi: 10.3109/02688690903386991. PubMed PMID: 20158353
https://doi.org/10.3109/02688690903386991
PMid:20158353Azzam D, Romiyo P, Nguyen T, Sheppard JP, Alkhalid Y, Lagman C, Prashant GN, Yang I Dural Repair in Cranial Surgery Is Associated with Moderate Rates of Complications with Both Autologous and Nonautologous Dural Substitutes. World Neurosurg. 2018; 113:244-248. doi: 10.1016/j.wneu.2018.01.115
https://doi.org/10.1016/j.wneu.2018.01.115
PMid:29374609Арутюнов, А.И., Н.Ш. Месхия. Некоторые актуальные вопросы пластики дефектов твёрдой мозговой оболочки. Вопросы нейрохирургии. 1972; 3: 3-9 Kizmazoglu C, Aydin HE, Kaya I, Atar M, Husemoglu B, Kalemci O, Sozer G, Havitcioglu H Comparison of Biomechanical Properties of Dura Mater Substitutes and Cranial Human Dura Mater : An In Vitro Study J Korean Neurosurg Soc. 2019; 62(6):635-642. doi: 10.3340/jkns.2019.0122. PMID: 31679317 PMCID: PMC6835148
https://doi.org/10.3340/jkns.2019.0122
PMid:31679317 PMCid:PMC6835148Rosen CL, Steinberg GK, DeMonte F, Delashaw JB Jr, Lewis SB, Shaffrey ME, Aziz K, Hantel J, Marciano FF. Results of the prospective, randomized, multicenter clinical trial evaluating a biosynthesized cellulose graft for repair of dural defects. Neurosurgery. 2011; 69 (5): 1093-103; discussion 1103-4. doi: 10.1227/NEU.0b013e3182284aca. PubMed PMID: 21670715
https://doi.org/10.1227/NEU.0b013e3182284aca
PMid:21670715Sandoval-Sanchez JH, Ramos-Zuniga R, de Anda SL, et al. A new bilayer chitosan scaffolding as a dural substitute: experimental evaluation. World neurosurgery. 2012; 77(3-4):577-582. doi:10.1016/j.wneu.2011.07.007
https://doi.org/10.1016/j.wneu.2011.07.007
PMid:22120335Lam FC, Kasper E. Augmented autologous pericranium duraplasty in 100 posterior fossa surgeries–a retrospective case series. Neurosurgery. 2012;71(2 Suppl Operative):ons302‐ doi:10.1227/NEU.0b013e31826a8ab0
https://doi.org/10.1227/NEU.0b013e31826a8ab0
PMid:22843136Hoover DA, Mahmood A. Ossification of autologous pericranium used in duraplasty. Case report. J Neurosurg. 2001; 95(2):350‐ doi:10.3171/jns.2001.95.2.0350
https://doi.org/10.3171/jns.2001.95.2.0350
PMid:11780910Stevens EA, Powers AK, Sweasey TA, Tatter SB, Ojemann RG. Simplified harvest of autologous pericranium for duraplasty in Chiari malformation Type I. Technical note. J Neurosurg Spine. 2009;11(1):80‐ doi:10.3171/2009.3.SPINE08196
https://doi.org/10.3171/2009.3.SPINE08196
PMid:19569946Warren WL, Medary MB, Dureza CD, et al. Dural repair using acellular human dermis: experience with 200 cases: technique assessment. Neurosurgery. 2000;46(6):1391‐ doi:10.1097/00006123-200006000-00020
https://doi.org/10.1097/00006123-200006000-00020
PMid:10834644Verlee A, Mincke S, Stevens CV. Recent developments in antibacterial and antifungal chitosan and its derivatives. Carbohydr Polym. 2017;164:268‐ doi:10.1016/j.carbpol.2017.02.001
https://doi.org/10.1016/j.carbpol.2017.02.001
PMid:28325326Масленнікова Л.Д. Полімерні композити. 2011. Київ. 300. Guo W, Guo Q, Zhang S, Li J: Manufacturing of artificial dura mater with chitosan polylactic acid. Chin J Clin Rehabil 9:24-25, 2005 Mengistu Lemma, S., F. Bossard, and M. Rinaudo, Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly (ethylene oxide) . International journal of molecular sciences, 2016. 17 (11). pii: E1790
https://doi.org/10.3390/ijms17111790
PMid:27792192 PMCid:PMC5133791Reed AM, Gilding DK. Biodegradable polymers for use in surgery-Poly(ethylene oxide)/poly(ethylene terephthalate) (PEO/PET) copolymers: 2. In vitro degradation. Polymer 1981, 22, 499-504
https://doi.org/10.1016/0032-3861(81)90169-5Kumari A, Yadav SK, Yadav SC. Biodegradable polymeric nanoparticles based drug delivery systems. Colloids Surf B Biointerfaces. 2010;75(1):1‐ doi:10.1016/j.colsurfb.2009.09.001
https://doi.org/10.1016/j.colsurfb.2009.09.001
PMid:19782542Zivanovic S, Li J, Davidson PM, Kit K. Physical, mechanical, and antibacterial properties of chitosan/PEO blend films. Biomacromolecules. 2007;8(5):1505‐ doi:10.1021/bm061140p
https://doi.org/10.1021/bm061140p
PMid:17388625Basu A, Krady JK, O’Malley M, Styren SD, DeKosky ST, Levison SW. The type 1 interleukin-1 receptor is essential for the efficient activation of microglia and the induction of multiple proinflammatory mediators in response to brain injury. J Neurosci. 2002;22(14):6071‐ doi:10.1523/JNEUROSCI.22-14-06071.2002
https://doi.org/10.1523/JNEUROSCI.22-14-06071.2002
PMid:12122068 PMCid:PMC6757935Dimzon IK, Knepper TP. Degree of deacetylation of chitosan by infrared spectroscopy and partial least squares. Int J Biol Macromol. 2015;72:939‐ doi:10.1016/j.ijbiomac.2014.09.050
https://doi.org/10.1016/j.ijbiomac.2014.09.050
PMid:25316417Kumirska J, Czerwicka M, Kaczyński Z, et al. Application of spectroscopic methods for structural analysis of chitin and chitosan. Mar Drugs. 2010;8(5):1567‐ Published 2010 Apr 29. doi:10.3390/md8051567
https://doi.org/10.3390/md8051567
PMid:20559489 PMCid:PMC2885081Paluszkiewicz C, Stodolak E, Hasik M, Blazewicz M. FT-IR study of montmorillonite-chitosan nanocomposite materials. Spectrochim Acta A Mol Biomol Spectrosc. 2011;79(4):784‐ doi:10.1016/j.saa.2010.08.053
https://doi.org/10.1016/j.saa.2010.08.053
PMid:20864391Park, H.J., Lee, J.S., Lee, O.J. et al. Fabrication of microporous three-dimensional scaffolds from silk fibroin for tissue engineering. Res. 2014; 22, 592-599. doi.org/10.1007/s13233-014-2083-0
https://doi.org/10.1007/s13233-014-2083-0Yiin-Kuen Fuh, Shengzhan Chen & Jason S.C. Jang. Direct-write, Well-aligned Chitosan-Poly (ethylene oxide) Nanofibers Deposited via Near-field Electrospinning, Journal of Macromolecular Science, Part A: Pureand Applied Chemistry. 2012; 49:10, 845-850, doi: 10.1080/10601325.2012.714676
https://doi.org/10.1080/10601325.2012.714676Svetlana Zivanovic, Jiajie Li, P. Michael Davidson and Kevin Kit. Physical, Mechanical, and Antibacterial Properties of Chitosan/PEO Blend Films. Biomacromolecules. 2007; 8,1505-1510. org/10.1021/bm061140p
https://doi.org/10.1021/bm061140p
PMid:17388625Katalinich, M. Characterization of Chitosan Films for Cell CultureApplications; The University of Maine, 2001 Foster LJ, Ho S, Hook J, Basuki M, Marçal H. Chitosan as a Biomaterial: Influence of Degree of Deacetylation on Its Physiochemical, Material and Biological Properties. PLoS One. 2015;10(8):e0135153. Published 2015 Aug 25. doi:10.1371/journal.pone.0135153
https://doi.org/10.1371/journal.pone.0135153
PMid:26305690 PMCid:PMC4549144Pre-Clinical Evaluation of Collagen Dura Substitutes in a Rabbit Duraplasty Model: DuraMatrix. 2015.
Panteleichuk A., Kadzhaya M, Biloschytsky V, Shmeleva A, Petriv T , Gnatyuk O, Dovbeshko G, Kozakevych R, Tyortyh V. Composite chitosan/polyethylene oxide film for duraplasty in traumatic brain injury model in rats. Cell Organ Transpl. 2020; 8(1):26-31. doi:10.22494/cot.v8i1.105
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