Composite chitosan/polyethylene oxide film for duraplasty in traumatic brain injury model in rats

Home/2020, Vol. 8, No. 1/Composite chitosan/polyethylene oxide film for duraplasty in traumatic brain injury model in rats

Cell and Organ Transplantology. 2020; 8(1):26-31.
DOI: 10.22494/cot.v8i1.105

Composite chitosan/polyethylene oxide film for duraplasty in traumatic brain injury model in rats

Panteleichuk A.¹, Kadzhaya M.¹, Biloschytsky V.2, Shmeleva A.3, Petriv T.4, Gnatyuk O.5, Dovbeshko G.5, Kozakevych R.6, Tyortyh V.6

  • 1Department of neurotrauma, Romodanov State Institute of Neurosurgery of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • 2Chronic pain treatment group, Romodanov State Institute of Neurosurgery of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • 3Department of Neuropatomorphology, Romodanov State Institute of Neurosurgery of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • 4Department of Reconstructive Neurosurgery with X-ray surgery, Romodanov State Institute of Neurosurgery of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • 5Institute of Physics of the National Academy of Sciences of Ukraine, Kyiv,  Ukraine
  • 6Chuiko Institute of Surface Chemistry of the National Academy of Sciences of Ukraine, Kyiv, Ukraine


The duraplasty is a standard procedure during neurosurgery for injuries and diseases of the brain. The hermetic closure of the dura mater (DM) not always possible with the application of autologous tissues. Synthetic, allogeneic and xenogenic implants, which are currently used, have disadvantages, so the search for the material that would best meet the requirements for a DM scaffold continues.
The purpose is to study the physical and chemical properties of the composite chitosan/polyethylene oxide (PEO) film and determine the effectiveness of its application for duraplasty in the experiment in vivo; to analyze its ability to biodegradation; to evaluate the effect of chitosan/PEO scaffold on the regeneration of dura matter.
Materials and methods. The experiment used 10 white rats aged 12 months with a penetrating traumatic brain injury model. Postoperative material was examined by macroscopy, optical microscopy and infrared spectroscopy.
Results. According to the analysis of infrared absorption, spectral markers of scar tissue, regenerating DM and intact DM were determined. Oscillation spectroscopy data indicate degradation of the chitosan film and repair of normal DM. Histology data also indicate biological degradation of the chitosan film and its replacement by newly formed normal connective tissue.
Conclusion. The data of morphological and spectroscopic studies show the ability of chitosan/PEO film to biodegradation in vivo with followed replacement not by scar but by normal connective tissue.

Key words: chitosan; polyethylene oxide; penetrating traumatic brain injury; infrared spectroscopy; morphological studies


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Panteleichuk A., Kadzhaya M, Biloschytsky V, Shmeleva A, Petriv T , Gnatyuk O, Dovbeshko G, Kozakevych R, Tyortyh V. Composite chitosan/polyethylene oxide film for duraplasty in traumatic brain injury model in rats. Cell Organ Transpl. 2020; 8(1):26-31. doi:10.22494/cot.v8i1.105

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