Cell and Organ Transplantology. 2020; 8(1):13-19.
The blood cells count in leukocyte and leukocyte-poor platelet-concentrated plasma in patients with musculoskeletal disorders
- State Institute of Traumatology and Orthopedics of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
The cellular composition significantly affects the properties of platelet concentrates. In particular, leukocyte platelet concentrates due to the increased number of monocytes and granulocytes have increased levels of proinflammatory cytokines, which contribute to the destruction of extracellular matrix, reduce synthesis of its components and enhance inflammation in tissues.
The purpose. To determine the blood cells number in leukocyte (L-PCP) or leukocyte-poor platelet-concentrated plasma (LP-PCP) and compare it with whole venous blood, plasma and platelet-poor plasma.
Materials and methods. 20 L-PCP and 21 LP-PCP samples were obtained by double centrifugation from the venous blood of 30 donors aged 26 to 78 with local pathology of the musculoskeletal system. In the first stage, plasma was isolated after the separation of whole blood, and in the second stage, platelets were concentrated in a small volume of platelet-poor plasma. The number of blood cells in the test samples was determined by hematology analyzer.
Results. When comparing the platelet count in L-PCP and LP-PCP, no significant difference was found in platelet number (2803.2 ± 933.8·106 and 2260 ± 943.9·106 cells/mL, respectively). The efficiency of platelet concentration from the initial volume of whole blood for the methods of obtaining LP-PCP and L-PCP did not differ significantly and was 76.5 ± 23.4 % and 83 ± 24.9 %, respectively. Platelet count in L-PCP was 8, 13 and 239 times higher than in plasma, whole blood and platelet-poor plasma, respectively. While the number of platelets in LP-PCP exceeded their count in the same samples by 8, 11 and 194 times, respectively. The leukocyte number in leukocyte-poor and leukocyte plasma with platelet concentrate was 2.5 ± 1.2·106 and 5.9 ± 1.8·106 cells/mL, respectively. However, their number in LP-PCP was almost 2 times lower than in blood and 5 times higher compared with plasma. The number of leukocytes in L-PCP was 30 % higher than in the blood, and 6 times higher than in plasma. The erythrocyte number was reduced by 99 % in both types of plasma with platelet concentrate compared with their count in whole blood.
Conclusions. According to the parameter of platelet isolation from whole blood, the procedures for obtaining leukocyte or leukocyte-poor platelet-concentrated plasma belong to the methods with medium efficiency. The procedure for preparing L-PCP allows to get a product with an increased relative number of leukocytes. For both studied products it was possible to achieve a significant reduction in erythrocyte count.
Key words: platelet concentrates; platelet-rich plasma; platelet-concentrated plasmaFull Text PDF (eng) Full Text PDF (ua)
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