Placental stem cells, organotypic culture and human placenta extract have neuroprotective activity in vitro

Home/2017, Vol. 5, No. 1/Placental stem cells, organotypic culture and human placenta extract have neuroprotective activity in vitro

Cell and Organ Transplantology. 2017; 5(1):39-42.
DOI: 10.22494/COT.V5I1.67

Placental stem cells, organotypic culture and human placenta extract have neuroprotective activity in vitro

Prokopyuk V. Yu., Chub O. V., Shevchenko M. V., Prokopyuk O. S.
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

According to WHO, 6.7 million people die from stroke every year. The search for new neuroprotective substances remains an urgent task.
The purpose of this study was to investigate the neuroprotective activity of factors of placental origin.
Materials and methods. Neuroprotective activity of media conditioned with cryopreserved placenta derived mesenchymal stem cells (MSCs), organotypic culture of placenta and placental extract was studied on in vitro models of glutamate excitotoxicity in rats` neural cells. Neural cells were cultured with placental factors without glutamate treatment, before and after glutamate treatment. Neural cells` metabolic activity was assessed by MTT test.
Results. Placental factors increase the MTT test indexes, prevent the toxic effect of glutamate on neural cells and promote their recovery. The thermolability of factors of placental origin and the effectiveness of various placental preparations are shown.
Conclusions. Conditional media of placenta derived MSCs, organotypic culture of the placenta and human placental extract have neuroprotective effect on rats` brain cells in vitro.

Key words: placenta; mesenchymal stem cells; placental extract; neural cells; neuroprotective effect

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1. Prokopyuk A, Shevchenko NA, Prokopyuk VYu, et al. Vpliv krіokonservovanih bіoobʼєktіv placentarnogo pohodzhennja na kul’turu klіtin [Influence of Cryopreserved Bioobjects of Placental Origin on Cell Culture]. Vіsnik problem bіologії і medicini − Bulletin of problems in biology and medicine. 2015; 1(122): 160-164. [In Ukrainian].
2. Benedusi V, Meda C, Sara D, et al. A lack of ovarian function increases neuroinflammation in aged mice. Endocrinology. 2012; 153(6): 2777–2788.
PMid:22492304 PMCid:PMC3359599
3. Chen J, Shehadah A, Pal A, et al. Neuroprotective effect of human placenta-derived cell treatment of stroke in rats. Cell Transplant. 2013; 22(5): 871-879.
4. Cizkova D, Devaux S, Le Marrec-Croq F, et al. Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study. Sci. Rep. 2014; doi:10.1038/srep07514
5. Gervois P, Wolfs E, Ratajczak J, et al. Stem cell-based therapies for ischemic stroke: preclinical results and the potential of imaging-assisted evaluation of donor cell fate and mechanisms of brain regeneration. Med Res Rev. 2016; 36(6): 1080-126.
6. Fukui M, Song JH, Choi J, et al. Mechanism of glutamate-induced neurotoxicity in HT22 mouse hippocampal cells. European Journal of Pharmacology. 2009; 619(1–3): 1-11.
7. Honmou O, Onodera R, Sasaki M, et al. Mesenchymal stem cells: therapeutic outlook for stroke. Trends Mol Med. 2012; 18(5): 292-97.
8. Hsuan YC, Lin CH, Chang CP, et al. Mesenchymal stem cell-based treatments for stroke, neural trauma, and heat stroke. Brain Behav. 2016; 6(10): e00526.
PMid:27781140 PMCid:PMC5064338
9. Kanno H, Kawakami Z, Mizoguchi K, et al. Kampo medicine, protects PC12 cells from glutamate induced death by augmenting gene expression of cystine glutamate antiporter system. PLoS One. 2014; 9(12): e116275.
PMid:25551766 PMCid:PMC4281137
10. Pogozhykh D, Prokopyuk V, Pogozhykh O, et al. Influence of factors of cryopreservation and hypothermic storage on survival and functional parameters of multipotent stromal cells of placental origin. PLoS One. 2015; 10(10): 1-25.
PMid:26431528 PMCid:PMC4592233
11. Pogozhykh D, Pogozhykh O, Prokopyuk V, et al. Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells. Stem Cell Research & Therapy. 2017; 8: 66.
PMid:28284229 PMCid:PMC5346212
12. Prokopyuk VYu, Prokopyuk OS, Musatova IB, et al. Safety of placental, umbilical cord and fetal membrane explants after cryopreservation. Cell and Organ Transplantology. 2015; 3(1): 34-38.
13. Schevchenko NO, Somova KV, Volina VV, et al. Dynamics of activity and duration of functioning of cryopreserved cryoextract, placental cells and fragments in the organism of experimental animals. Morphologia. 2016; 10(2): 93-98.
14. Sukach AN, Shevchenko MV, Liashenko TD. Comparative study on influence of fetal bovine serum and serum of adult rat on cultivation of newborn rat neural cells. Biopolymers and cell. 2014; 30(5): 394-99.
15. Takuma K, Mizoguchi H, Funatsu Y, et al. Placental extract improves hippocampal neuronal loss and fear memory impairment resulting from chronic restraint stress in ovariectomized mice. J Pharmacol Sci. 2012; 120: 89-97.
16. Wan Z, Mah D, Simtchouk S, et al. Human adipose tissue conditioned media from lean subjects is protective against H2O2 induced neurotoxicity in human SH-SY5Y neuronal cells. Int J Mol Sci. 2015; 16(1): 1221-31.
PMid:25569096 PMCid:PMC4307300
17. The top 10 causes of death. Available:
18. Zhao L, Brinton RD. Select estrogens within the complex formulation of conjugated equine estrogens (Premarin ®) are protective against neurodegenerative insults: implications for a composition of estrogen therapy to promote neuronal function and prevent Alzheimer’s disease. BMC Neuroscience. 2006; 7: 24.
19. Zhi L, Zhao W, Liu W, et al. Transplantation of placenta-derived mesenchymal stem cell-induced neural stem cells to treat spinal cord injury. Neural Regen Res. 2014; 9(24): 2197-2204.
PMid:25657742 PMCid:PMC4316454

Prokopyuk VYu, Chub OV, Shevchenko MV, Prokopyuk OS. Placental stem cells, organotypic culture and human placenta extract have neuroprotective activity in vitro. Cell and Organ Transplantology. 2017; 5(1):39-42. doi:10.22494/cot.v5i1.67


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