Isolation of nucleated cells fraction from umbilical cord blood - choice of method

Home/2014, Vol. 2, No. 1/Isolation of nucleated cells fraction from umbilical cord blood – choice of method

Cell and Organ Transplantology. 2014; 2(1):30-33.
DOI: 10.22494/COT.V2I1.34

Isolation of nucleated cells fraction from umbilical cord blood – choice of method

Ivolgin D. A.1, 2, Smolyaninov A. B.1, 2
1Research laboratory of cell technologies of the Northwest State Medical University I.I. Mechnikov, St. Petersburg , Russian Federation
2Pokrovsky stem cell bank, St. Petersburg, Russian Federation

Abstract
The comparison of different techniques for cord blood processing to ensure the highest cell yield based on the “cost-effectiveness” ratio is important.
A study of the effectiveness of nucleated cells fraction isolation from umbilical cord blood samples (n = 2898) using double centrifugation, automatic (Sepax S100, Biosafe) or semi-automatic (MacoPress Smart, MacoPharma) system was performed. These include the determining of nucleated cells number (per ml and total), the number of CD34+ cells, and cell viability before samples treatment, after treatment and before freezing.
It was shown that semi-automatic and automatic processing methods were more efficient then the double centrifugation by key quality indicators of cell concentrates: the total number of nucleated cells and nucleated cells output. The semi-automatic method of nucleated cells fraction isolation requires the least time for processing a single sample.
Considering these data such a system has been proposed as optimal for daily receipt and processing of cord blood samples in a cord blood bank.

Keywords: umbilical cord blood, cord blood bank, cell separation semi-automated system

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Ivolgin DA, Smolyaninov AB. Isolation of nucleated cells fraction from umbilical cord blood – choice of method. Cell and Organ Transplantology. 2014; 2(1):30-33. doi: 10.22494/COT.V2I1.34

 

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