Use of human fetal liver cells for treatment of patients with lower limb peripheral artery disease

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Cell and Organ Transplantology. 2014; 2(1):10-13.
DOI: 10.22494/COT.V2I1.39

Use of human fetal liver cells for treatment of patients with lower limb peripheral artery disease

Salyutin R. V., Dombrowski D. B., Komarov M. P., Sokolov N. F., Palyanitsya S. S., Shabliy V. A.
A. A. Shalimov National Institute of Surgery and Transplantology of NAMS Ukraine, Kyiv, Ukraine
Coordinating Centre of Transplantation for Organs, Tissue and Cells, Health Ministry of Ukraine, Kyiv, Ukraine
Institute of Cell Th erapy, Kyiv, Ukraine

Abstract
In the group of patients (n = 21, mean age 54 ± 5.8 years) with chronic lower limb ischemia stage IIB who were non-liable for reconstructiverestoration surgery, we have established positive clinical effects of local transplantation of human fetal liver progenitor cells. Complex examination following 1, 3, 6 and 12 months after transplantation included duplex scanning of limb arteries, x-ray contrast arteriography and laser Doppler flowmetry as well as measuring pain-free walking and evaluating life quality based on individual questionnaire data.
Owing to the transplant “Cryopreserved human fetal liver progenitor cells” the patients demonstrated stable increase of life quality index and pain-free walking as well as improvement of general health allowing assign them to the group of patients with lower ischemia stage,  quicker social rehabilitation and lesser risk of disabling surgery (р < 0.05). Also, there were observations of improved microcirculation in the ischemic extremities owing to activation of endothelium-independent mechanisms of vasodilatation, reduced myotonus and neurotonus of the pre-capillaries and improved endothelium-dependent influence on the microhaemodynamic and, hence, an increased reserve capillary blood flow (p < 0.05).
Analysis of the obtained results indicates prospects and effectiveness of using fetal liver cells transplantation in the patients who are not liable for surgical reconstruction of the vascular bed.

Key words: chronic lower extremity ischemia, stem cells, fetal liver, cell transplantation.

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1. Faglia E, Clerici G, Airoldi F, et al. Revascularization by Angioplasty of Type D Femoropopliteal and Long Infrapopliteal Lesion in Diabetic Patients With Critical Limb Ischemia: Are TASC II Recommendations Suitable? A Population-Based Cohort Study. International Journal of Lower Extremity Wounds, December. 2012; 11:277 – 285.
https://doi.org/10.1177/1534734612463701
PMid:23089965
2. Voronov DA, Gavrylenko AV, Bochkov NP. Stimuljacija angiogeneza v uluchshenii rezul’tatov hirurgicheskogo lechenija pacientov s hronicheskoj ishemiej nizhnih konechnostej: jeksperimental’noe obosnovanie i jeffektivnost’ klinicheskogo primenenija [Stimulation of angiogenesis to improve the results of surgical treatment of patients with chronic lower limb ischemia: an experimental study and clinical application efficiency]. Bolezni aorty i ee vetvej – Diseases of the aorta and its branches. 2009; 3:45 – 48.
3. Smoljaninov AB, Pihtin EV, Bulgin DV, Tomonaga M. Kletochnye tehnologii v lechenii terminal’noj stadii hronicheskoj ishemii nizhnih konechnostej [Cell technologies in the treatment of end-stage chronic lower limb ischemia] Kletochnaja transplantalogija i tkanevaja inzhenerija – Cell and Tissue Engineering transplantology. 2007; 3:40 – 46
4. Jonsson TB. Adverse events during treatment of critical limb ischemia with autologous peripheral blood mononuclear cell implant. Int Angiol. 2012; 31(1):77 – 84.
PMid:22330628
6. Voronov DA, Gavrylenko AV Bochkov NP. Stimuljacija angiogeneza v uluchshenii rezul’tatov hirurgicheskogo lechenija pacientov s hronicheskoj ishemiej nizhnih konechnostej: jeksperimental’noe obosnovanie i jeffektivnost’ klinicheskogo primenenija [Stimulation of angiogenesis to improve the results of surgical treatment of patients with chronic lower limb ischemia: an experimental study and clinical application efficiency]. Bolezni aorty i ee vetvej – Diseases of the aorta and its branches. 2009; 3:45 – 48.
7. Grin VK, Shutin AA, Popadopulo AG, Basatsky AV, Varshaver PL. Autotransplantacija stromal’nyh stvolovyh kletok v lechenii obliterirujushhih zabolevanij arterij nizhnih konechnostej [Autografting stromal stem cells in the treatment of occlusive arterial disease of the lower limbs]. Vestnik neotlozhnoj i vosstanovitel’noj mediciny – Bulletin of emergency and rehabilitation medicine. 2010; 11(4): 512 – 513.
8. Benoit E, O’Donnell TF, Iafrati MD, Asher E, Bandyk DF. The role of amputation as an outcome measure in cellular therapy for critical limb ischemia: implications for clinical trial design. Journal of Translational Medicine. 2011; 9:165 – 174.
https://doi.org/10.1186/1479-5876-9-165
PMid:21951607 PMCid:PMC3191337
9. Walter DH. Intraarterial administration of bone marrow mononuclear cells in patients with critical limb ischemia: a randomized-start, placebo-controlled pilot trial (PROVASA). Circ Cardiovasc Interv. 2011, 4(1):26 – 37.
https://doi.org/10.1161/CIRCINTERVENTIONS.110.958348
PMid:21205939
10. Kuharchuk AL, Radchenko VV, Sirman VM. Stvolovye kletki: eksperiment, teorija, klinika. Jembrional’nye, mezenhimal’nye, nejral’nye i gemopojeticheskie stvolovye kletki [Stem cells: experiment, theory, clinic. Embryonic, mesenchymal, neural and hematopoietic stem cells]. Chernovcy: Zoloti litavry, 2004; 505 p.
11. Norgren L, Hiatt W R, Dormandy J A, Nehler M R, Harris K A, Fowkes FG, et al. Inter-Society Consensus for the Management of Peripheral Arterial Disease (TASC II). Eur J Vasc Endovasc Surg. 2007; 33(1):1 – 75.
https://doi.org/10.1016/j.ejvs.2006.09.024
PMid:17140820
12. Dormandy JA, Rutherford RB. Management of peripheral arterial disease (PAD). TASC Working Group. TransAtlantic Inter-Society Consensus (TASC). J Vasc Surg. 2000; 31(1 Pt 2):S1 – S296.
PMid:10666287

Salyutin RV, Dombrowski DB, Komarov MP, Sokolov NF, Palyanitsya SS, Shabliy VA. Use of human fetal liver cells for treatment of patients with lower limb peripheral artery disease. Cell and Organ Transplantology. 2014; 2(1):10-13. doi: 10.22494/COT.V2I1.39

 

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