Cryopreserved autologous multipotent mesenchymal stromal cells in the treatment of experimental tendopathy

Home/2014, Vol. 2, No. 1/Cryopreserved autologous multipotent mesenchymal stromal cells in the treatment of experimental tendopathy

Cell and Organ Transplantology. 2014; 2(1):62-67.
DOI: 10.22494/COT.V2I1.42

Cryopreserved autologous multipotent mesenchymal stromal cells in the treatment of experimental tendopathy

Volkovа N. A., Yukhta M. S., Вlonskiy R. I.*, Kostrub A. A.*, Goltsev A. N.
Institute of Рroblems of Cryobiology and Сryomedicine of NAS of Ukraine, Kharkiv, Ukraine
*Institute of Traumatology and Orthopedics of NAMS of Ukraine, Kiev, Ukraine

Abstract
To date, stem cells application is one of the promising methods to treat pathologies of the musculoskeletal system.
Material and methods. On the model of Achilles tendon degenerative injuries in rats (n = 60) we studied the effectiveness of local and systemic administration of cryopreserved autologous bone marrow multipotent mesenchymal stromal cells (MMSCs). We analyzed the morphology of the tissue, collagen type I content and the presence of labeled РКН-26 cells. Also the biomechanical study was performed on the 7th, 21st and 45th day after transplantation.
Results. It was shown that MMSCs contribute to the activation of regenerative processes in damaged tendons that was manifested in the recovery of histological structure, strength and type I collagen content. Local method of cell administration resulted in more pronounced tendon recovery as compared to systemic method. Using РКН-26 we confirmed the presence of injected cells in damaged area within 21 days.
Conclusion. The results of the study can be used for argumentation and development of methods for the treatment of degenerative and dystrophic tendon damages in clinical practice.

Keywords: cells cryopreservation, multipotent mesenchymal stromal cells, cell therapy, tendopathy

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Volkovа NA, Yukhta MS, Вlonskiy RI, Kostrub AA, Goltsev AN. Cryopreserved autologous multipotent mesenchymal stromal cells in the treatment of experimental tendopathy. Cell and Organ Transplantology. 2014; 2(1):62-67. doi: 10.22494/COT.V2I1.42

 

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