Cryopreserved autologous multipotent mesenchymal stromal cells in the treatment of experimental tendopathy

Home/2014, Vol. 2, No. 1/Cryopreserved autologous multipotent mesenchymal stromal cells in the treatment of experimental tendopathy

Cell and Organ Transplantology. 2014; 2(1):62-67.
DOI: 10.22494/COT.V2I1.42

Cryopreserved autologous multipotent mesenchymal stromal cells in the treatment of experimental tendopathy

Volkovа N. A., Yukhta M. S., Вlonskiy R. I.*, Kostrub A. A.*, Goltsev A. N.
Institute of Рroblems of Cryobiology and Сryomedicine of NAS of Ukraine, Kharkiv, Ukraine
*Institute of Traumatology and Orthopedics of NAMS of Ukraine, Kiev, Ukraine

To date, stem cells application is one of the promising methods to treat pathologies of the musculoskeletal system.
Material and methods. On the model of Achilles tendon degenerative injuries in rats (n = 60) we studied the effectiveness of local and systemic administration of cryopreserved autologous bone marrow multipotent mesenchymal stromal cells (MMSCs). We analyzed the morphology of the tissue, collagen type I content and the presence of labeled РКН-26 cells. Also the biomechanical study was performed on the 7th, 21st and 45th day after transplantation.
Results. It was shown that MMSCs contribute to the activation of regenerative processes in damaged tendons that was manifested in the recovery of histological structure, strength and type I collagen content. Local method of cell administration resulted in more pronounced tendon recovery as compared to systemic method. Using РКН-26 we confirmed the presence of injected cells in damaged area within 21 days.
Conclusion. The results of the study can be used for argumentation and development of methods for the treatment of degenerative and dystrophic tendon damages in clinical practice.

Keywords: cells cryopreservation, multipotent mesenchymal stromal cells, cell therapy, tendopathy

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1. Adams R. Methods of culture of cells for biochemists. Elsevier/North-Holland Biomedical Press, 1980; 292 p. [Russ. ed.: Metody kul'tury kletok dlja biohimikov. Moscow: Mir, 1983; 264 p].
2. Volkova NO, Kostrub OO, Blonsky RІ, et al. Klitynni kul’tury stromal’nogo pohodzhennja v terapii’ eksperymental’noi’ tendopatii’ [Stromal cell cultures in the therapy of an experimental tendopatiya]. Ortopedija, travmatologija ta protezuvannja – Orthopaedics, Traumatology and Prosthetics, 2012;4:40-44.
5. Kostrub OO, Blonsky RI, Lazarev IA, et al. Micnist’ suhozhyllja na roztjaguvannja pislja klitynnoi’ terapii’ jogo degeneratyvnogo poshkodzhennja v eksperymenti [The strength of the tendon to stretch after his stem cell therapy of degenerative lesions in experimental]. Visnyk ortopedii’, travmatologii’ ta protezuvannja – Bulletin of orthopedics, traumatology and prosthetics, 2011;3:23-26.
6. Muldashev ER, Nigmatullin RT, Gafarov VG, et al. Vosstanovlenie struktury suhozhilij s primeneniem biomaterialov Alloplant [Restoration of the structure of tendons using biomaterials Alloplant] Regenerativnaja hirurgija – The regenerative surgery, 2005;1:56-61.
7. Rompe JD, Fury D, Maffulli N. Travmatizm suhozhilij i metody vosstanovitel’noj terapii [The injuries of tendons and methods of rehabilitation therapy]. Zhurnal kostno-sustavnoj hirurgii – The Journal of bone and joint surgery, 2008;3:15-19.
8. Alhadlaq A. Mesenchymal stem cells: isolation and therapeutics. Stem. Cells. Dev. 2004;13(4):436–448.
9. Anjos-Afonso F, Siapati EK, Bonnet D. In vivo contribution of murine mesenchymal stem cells into multiple cell-types under minimal damage conditions. J Cell. Sci. 2004; 117:5655-5664.
10. Awad HA, Butler DL, Boivin GP, et al. Autologous mesenchymal stem cell-mediated repair of tendon. Tissue Eng. 1999;5:267–277.
11. Bittencourt R, Pereira H, Felisbino S, et al. Isolation of bone marrow mesenchymal stem cells. Acta Ortop. Bras. 2006;14:22-24.
12. Bossley ChJ, Martinelli B, Maffulli N, Raisbeck C. Rupture of the Achilles Tendon. J Bone Joint Surg Am. 2000;82:1804-1804.
13. Bhakta S, Hong P, Koc O. The surface adhesion molecule CXCR4 stimulates mesenchymal stem cell migration to stromal cell-derived factor-1 in vitro but does not decrease apoptosis under serum deprivation. Cardiovasc Revasc Med. 2006; 7(1):19-24.
14. Caplan AI. Mesenchymal stem cells: cell-based reconstructive therapy in orthopedics. Tissue Engineering. 2005; 11:1198–1211.
15. Graham R. Tendinopathy – from basic science to treatment. Nature Clinical Practice Rheumatology. 2008; 4:82-89.
16. Haack-Sorensen M, Bindslev L, Mortensen S, et al. The influence of freezing and storage on the characteristics and functions of human mesenchymal stromal cells isolated for clinical use. Cytotherapy. 2007; 9:328-337.
17. Jacobsson J. Towards systematic prevention of athletics injuries: Use of clinical epidemiology for Evidence based injury prevention. Linköping, Sweden, 2012. 71 p.
18. Karp JM, Leng GS. Mesenchymal stem cell homing: the devil is in the details. Stem Cell. 2009; 4:206-216.
19. Klingemann H, Matzilevich D, Marchand J. Mesenchymal stem cells –sources and clinical applications. Transfus. Med. Hemother. 2008; 35:272–277.
PMid:21512642 PMCid:PMC3076359
20. Krampera M, Cosmi L, Angeli R, et. al. Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells. Stem Cells. 2006; 24:386-398.
21. Le Blanc K, Mougiakakos D. Multipotent mesenchymal stromal cells and the innate immune system. Nat Rev Immunol. 2012; 12:383–396.
22. Lee JS, Hong JM, Moon GJ, et al. A long-term follow-up study of intravenous autologous mesenchymal stem cell transplantation in patients with ischemic stroke. Stem Cells. 2010; 28:1099–1106.
23. Molloy T., Wang Y., Murrell G. The roles of growth factors in tendon and ligament healing. Sports Medicine. 2003; 33:391-394.
24. Nourissat G, Diop A, Maurel N. Mesenchymal stem cell therapy regenerates the native bone-tendon junction after surgical repair in a degenerative rat model. PLoS ONE. 2010; 5:122-131.
PMid:20805884 PMCid:PMC2923611
25. Pacini S, Spinabella S, Trombi L, et. al. Suspension of bone marrow-derived undifferentiated mesenchymal stromal cells for repair of superficial digital flexor tendon in race horses. Tissue Eng. 2007; 13:2949-2955.
26. Qiu Y, Wang X, Zhang Y, et al. Development of a refined tenocyte differentiation culture technique for tendon tissue engineering. Cells Tissues Organs. 2013; 197:27–36.
27. Rees JD, Wilson AM, Wolman RL. Current concepts in the management of tendon disorders. Rheumatology. 2006; 45; 508–521.
28. Richardson LE, Dudhia J, Clegg PD, Smith R. Stem cells in veterinary medicine–attempts at regenerating equine tendon after injury. Trends Biotech. 2007; 25:409-416.
29. Ritty T, Ditsios K., Starcher B. Distribution of the elastic fiber and associated proteins in flexor tendon reflects function. Anat. Record. 2002; 268:430–440.
30. Södersten F, Hultenby K, Heinegаrd D, et al. Immunolocalization of collagens (I and III) and cartilage oligomeric matrix protein in the normal and injured equine superficial digital flexor tendon. Connective Tissue Research. 2013; 54:62–69.
PMid:23020676 PMCid:PMC3545546
31. Tabera S, Perez-Simon J.A, Diez-Campelo M. The effect of mesenchymal stem cells on the viability, proliferation and differentiation of B-lymphocytes. Haematologica. 2008; 93:1301-1309.
32. Zhao Ch, Chieh H, Bakri K, et al. The effects of bone marrow stromal cell transplants on tendon healing in vitro. Med. Eng. Physics. 2009; 31:1271–1275.
PMid:19736035 PMCid:PMC3898667

Volkovа NA, Yukhta MS, Вlonskiy RI, Kostrub AA, Goltsev AN. Cryopreserved autologous multipotent mesenchymal stromal cells in the treatment of experimental tendopathy. Cell and Organ Transplantology. 2014; 2(1):62-67. doi: 10.22494/COT.V2I1.42


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