Cellular immune response in rats with 1,2-dimethylhydrazine-induced colon cancer after transplantation of placenta-derived multipotent cells

Home/2016, Vol. 4, No. 1/Cellular immune response in rats with 1,2-dimethylhydrazine-induced colon cancer after transplantation of placenta-derived multipotent cells

Cell and Organ Transplantology. 2016; 4(1):55-60.
DOI: 10.22494/COT.V4I1.7

Cellular immune response in rats with 1,2-dimethylhydrazine-induced colon cancer after transplantation of placenta-derived multipotent cells

Svitina H.1,2, Kalmukova O.1, Shelest D.1, Skachkova O.3, Garmanchuk L.1, Shablii V.2
1Educational and Scientific Centre “Institute of Biology”, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
2Institute of Cell Therapy, Kyiv, Ukraine
3National Cancer Institute, Kyiv, Ukraine

Abstract
We describe the state of the immune system at the late stage of 1,2-dimethylhydrazine (DMH)-induced colon cancer and after administration of placenta-derived multipotent cells (PDMCs). The spleen and thymus indices did not differ among the groups of intact and DMH-treated rats and were not affected by the administration of placenta-derived multipotent cells following the DMH treatment. Moreover, no difference in spontaneous or stimulated phagocytic activity of peritoneal macrophages was observed between healthy rats or the animals with DMH-induced colon cancer (with or without the administration of PDMCs). However, the proliferation of the T cells in the spleen was lower in rats with colon cancer regardless of the administration of PDMCs. Similarly, no changes were observed in the cell cycle distribution of proliferating spleen cells after stimulation by lipopolysaccharide.
Conclusion. Our data demonstrate the absence of the active reaction by peritoneal macrophages and spleen cells to a colon cancer at mid/late stage. Additionally, the administration of PDMCs does not result in a measurable anti-tumor immune response.

Key words: 1,2-dimethylhydrazine, colon cancer, placenta-derived multipotent cells, immune response

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1. Krzystek-Korpacka M, Diakowska D, Kapturkiewicz B, et al. Profiles of circulating inflammatory cytokines in colorectal cancer (CRC), high cancer risk conditions, and health are distinct. Possible implications for CRC screening and surveillance. Cancer Lett. 2013; 337(1): 107-14.
https://doi.org/10.1016/j.canlet.2013.05.033
PMid:23726839
2. Lakatos L, Mester G, Erdelyi Z, et al. Risk factors for ulcerative colitis-associated colorectal cancer in a Hungarian cohort of patients with ulcerative colitis: results of a population-based study. Inflamm Bowel Dis. 2006; 12(3): 205-11.
https://doi.org/10.1097/01.MIB.0000217770.21261.ce
PMid:16534422
3. Lee WS, Baek JH, You DH, et al. Prognostic value of circulating cytokines for stage III colon cancer. J Surg Res. 2013; 182(1): 49-54.
https://doi.org/10.1016/j.jss.2012.08.051
PMid:23010514
4. Paludan C, Edinger JW, Harbacheuski R, Murray RA, Hariri RJ. Immunomodulation using placental stem cells. US Patent, no US 20150140044 A1, 2015.
5. Evangelista M, Soncini M, Parolini O. Placenta-derived stem cells: new hope for cell therapy? Cytotechnology. 2008; 58(1): 33-42.
https://doi.org/10.1007/s10616-008-9162-z
PMid:19002775 PMCid:PMC2593758
6. van der Bij GJ, Bögels M, Oosterling SJ, et al. Tumor infiltrating macrophages reduce development of peritoneal colorectal carcinoma metastases. Cancer Lett. 2008; 262(1): 77-86.
https://doi.org/10.1016/j.canlet.2007.11.040
PMid:18187256
7. Kruse J, von Bernstorff W, Evert K, et al. Macrophages promote tumour growth and liver metastasis in an orthotopic syngeneic mouse model of colon cancer. Int J Colorectal Dis. 2013; 28(10): 1337-49.
https://doi.org/10.1007/s00384-013-1703-z
PMid:23657400
8. Olszewski WL, Kubicka U, Tarnowski W, et al. Activation of human peritoneal immune cells in early stages of gastric and colon cancer. Surgery. 2007; 141(2): 212-21.
https://doi.org/10.1016/j.surg.2006.06.031
PMid:17263978
9. Cesta MF. Normal structure, function, and histology of the spleen. Toxicol Pathol. 2006; 34(5): 455-65.
https://doi.org/10.1080/01926230600867743
PMid:17067939
10. Mebius RE, Kraal G. Structure and function of the spleen. Nat Rev Immunol. 2005; 5(8): 606-16.
https://doi.org/10.1038/nri1669
PMid:16056254
11. Dunn GP, Bruce AT, Ikeda H, et al. Cancer immunoediting: from immunosurveillance to tumor escape. Nat Immunol. 2002; 3(11): 991-8.
https://doi.org/10.1038/ni1102-991
PMid:12407406
12. Scarpa M, Castagliuolo I, Castoro C, et al. Inflammatory colonic carcinogenesis: a review on pathogenesis and immunosurveillance mechanisms in ulcerative colitis. World J Gastroenterol. 2014; 20(22): 6774-85.
https://doi.org/10.3748/wjg.v20.i22.6774
PMid:24944468 PMCid:PMC4051917
13. Ugurel S, Uhlig D, Pföhler C, et al. Down-regulation of HLA class II and costimulatory CD86/B7-2 on circulating monocytes from melanoma patients. Cancer Immunol Immunother. 2004; 53: 551-59.
https://doi.org/10.1007/s00262-003-0489-1
PMid:14727087
14. Chouaib S, Asselin-Paturel C, Mami-Chouaib F, et al. The host-tumor immune conflict: from immunosuppression to resistance and destruction. Immunol Today. 1997; 18: 493 97.
https://doi.org/10.1016/S0167-5699(97)01115-8
15. Yuan W, Zong C, Huang Y, et al. Biological, immunological and regenerative characteristics of placenta-derived mesenchymal stem cell isolated using a time-gradient attachment method. Stem Cell Res. 2012; 9(2): 110-23.
https://doi.org/10.1016/j.scr.2012.05.003
PMid:22687723
16. Brooke G, Rossetti T, Pelekanos R, et al. Manufacturing of human placenta-derived mesenchymal stem cells for clinical trials. Br J Haematol. 2009; 144(4): 571-9.
https://doi.org/10.1111/j.1365-2141.2008.07492.x
PMid:19077161
17. Oliveira MS, Barreto-Filho JB. Placental-derived stem cells: Culture, differentiation and challenges. World J Stem Cells. 2015; 7(4): 769-75.
https://doi.org/10.4252/wjsc.v7.i4.769
PMid:26029347 PMCid:PMC4444616
18. Vellasamy S, Sandrasaigaran P, Vidyadaran S, et al. Isolation and characterisation of mesenchymal stem cells derived from human placenta tissue. World J Stem Cells. 2012; 4: 53 61.
https://doi.org/10.4252/wjsc.v4.i6.53
PMid:22993662 PMCid:PMC3443712
19. Perše M, Cerar A. Morphological and molecular alterations in 1,2 dimethylhydrazine and azoxymethane induced colon carcinogenesis in rats. J Biomed Biotechnol. 2011; 2011: 473964.
https://doi.org/10.1155/2011/473964
PMid:21253581 PMCid:PMC3018655
20. Chen JR, Yang ZQ, Hu TJ, et al. Immunomodulatory activity in vitro and in vivo of polysaccharide from Potentilla anserina. Fitoterapia. 2010; 81(8): 1117-24.
https://doi.org/10.1016/j.fitote.2010.07.009
PMid:20624446
21. Fang JJ, Zhu ZY, Dong H, et al. Effect of spleen lymphocytes on the splenomegaly in hepatocellular carcinoma-bearing mice. Biomed Environ Sci. 2014; 27(1): 17-26.
PMid:24553370
22. Sharp JG, Riches AC, Littlewood V, et al. The incidence, pathology and transplantation of hepatomas in CBA mice. J Pathol. 1976; 119(4): 211-20.
https://doi.org/10.1002/path.1711190405
PMid:182943
23. Toge T, Kuroi K, Kuninobu H, et al. Role of the spleen in immunosuppression of gastric cancer: predominance of suppressor precursor and suppressor inducer T cells in the recirculating spleen cells. Clin Exp Immunol. 1988; 74(3): 409-12.
PMid:2976622 PMCid:PMC1542017
24. Bilynskiy BT, Volodko NA, Shparyk YaV. Immunologicheskie mekhanizmy estestvennoy protivoopukholevoy rezistentnosti [Immunological mechanisms of natural antitumor resistance]. Kiev: Naukova dumka, 1991. 245 p. [in Russian].
25. Medzhitov R, Dzhanevey Ch. Vrozhdennyy immunitet [Natural immunity]. Kazanskiy med. zhurn. 2005; 3: 161-7 [in Russian].
26. Quail DF, Joyce JA. Microenvironmental regulation of tumor progression and metastasis. Nat Med. 2013; 19(11): 1423-37. doi: 10.1038/nm.3394
https://doi.org/10.1038/nm.3394
27. Luan X, Li G, Wang G, et al. Human placenta-derived mesenchymal stem cells suppress T cell proliferation and support the culture expansion of cord blood CD34⁺ cells: a comparison with human bone marrow-derived mesenchymal stem cells. Tissue Cell. 2013; 45(1): 32-8.
https://doi.org/10.1016/j.tice.2012.09.002
PMid:23107983
28. Liu KJ, Wang CJ, Chang CJ, et al. Surface expression of HLA-G is in;ved in mediating immunomodulatory effects of placenta-derived multipotent cells (PDMCs) towards natural killer lymphocytes. Cell Transplant. 2011; 20(11-12): 1721-30.
https://doi.org/10.3727/096368911X580590
PMid:21669042
29. Li CD, Zhang WY, Li HL, et al. Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation. Cell Res. 2005; 15(7): 539-47.
https://doi.org/10.1038/sj.cr.7290323
PMid:16045817
30. Zhu Y, Yang Y, Zhang Y, et al. Placental mesenchymal stem cells of fetal and maternal origins demonstrate different therapeutic potentials. Stem Cell Res Ther. 2014; 5(2): 48.
https://doi.org/10.1186/scrt436
PMid:24721710 PMCid:PMC4055134
31. Deryagina VP, Ryzhova NI, Golubeva IS. Phagocyte functional activity and production of nitric oxide compounds in mouse models of tumor xenografts. Vestnik RONTs im. N. N. Blokhina RAMN. 2011; 22(2): 49-57.
32. Kladnytska LV, Nikulina VV, Garmanchuk LV, et al. Influence Allogeneic Mesenchymal Stem Cells on the Tumour Growth Parameters and Metastatic Potential in the Transplantable Carcinoma Lung Lewis. Journal of Animal and Veterinary Sciences. 2014; 1(1): 1-5.
33. Kladnytska LV, Mazurkevych AY, Garmanchuk LV, et al. The biological properties of tumor cells in c57bl/6 mice with transplantable lewis lung carcinoma with influence of allogeneic mesenchymal stem cells. The Animal Biology. 2015; 17(2): 82-8.
34. Fang JJ, Zhu ZY, Dong H, et al. Effect of spleen lymphocytes on the splenomegaly in hepatocellular carcinoma-bearing mice. Biomed Environ Sci. 2014; 27(1): 17-26.
PMid:24553370

Svitina H, Kalmukova O, Shelest D, Skachkova O, Garmanchuk L, Shablii V.  Cellular immune response in rats with 1,2-dimethylhydrazine-induced colon cancer after transplantation of placenta-derived multipotent cells. Cell and Organ Transplantology. 2016; 4(1):55-60. doi: 10.22494/COT.V4I1.7

 

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