Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Home/2015, Vol. 3, No. 1/Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Cell and Organ Transplantology. 2015; 3(1):34-38.
DOI: 10.22494/COT.V3I1.18

Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Prokopyuk V. Yu., Prokopyuk O. S., Musatova I. B., Shevchenko N. А., Roenko A. А., Terehova E. A., Volina V. V.
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Abstract
There have been studied morphological safety and functional state of the explants of human placenta, umbilical cord and fetal membranes by vital staining techniques using the MTT and resazurin reduction tests, level of glucose in incubation medium, activity of lactate dehydrogenase and alkaline phosphatase before and after cryopreservation. It has been found that proposed program of cryopreservation allows keeping a high level of viability of the explants of placenta, umbilical cord and fetal membranes, the most informative methods of assessing the safety of these biological objects before and after cryopreservation are method of vital staining, determination of glucose content in incubation medium, MTT-test and resazurin reduction test.

Keywords: placenta; umbilical cord; fetal membrane explants; cryopreservation

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Prokopyuk VYu, Prokopyuk OS, Musatova IB., Shevchenko NА, Roenko AА, Terehova EA, Volina VV. Safety of placental, umbilical cord and fetal membrane explants after cryopreservation. Cell and Organ Transplantology. 2015; 3(1):34-38. doi: 10.22494/COT.V3I1.18

 

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