A protocol for isolation of fetal neural progenitor cells from mouse hippocampus

Home/2014, Vol. 2, No. 2/A protocol for isolation of fetal neural progenitor cells from mouse hippocampus

Cell and Organ Transplantology. 2014; 2(2):155-157.
DOI: 10.22494/COT.V2I2.32

A protocol for isolation of fetal neural progenitor cells from mouse hippocampus

Tsupykov О. М.
Bogomoletz Institute of Physiology NAS Ukraine, Кyiv, Ukraine
State Institute of Genetic and Regenerative Medicine NAMS Ukraine, Kyiv, Ukraine

Abstract
Culture of neural stem/progenitor cells are widely used to study the characteristics of these cells under controlled conditions in vitro as well as to study the cellular and molecular mechanisms of CNS diseases and develop strategies for their treatment.
This paper provides a detailed protocol to isolate of fetal (E17-18) neural progenitor cells (NPCs) of mouse hippocampus. The technique is based on the use of centrifugation of hippocampal cells suspension in Percoll density gradient to obtain purified NPCs fractions. The cells are cultured in serum-free medium in a monolayer, which creates conditions for more equitable access of FGF-2 to the cells. This method provides a homogeneous population of undifferentiated progenitors from fetal mouse hippocampus.

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Tsupykov ОМ. A protocol for isolation of fetal neural progenitor cells from mouse hippocampus. Cell and Organ Transplantology. 2014; 2(2):155-157. doi: 10.22494/COT.V2I2.32

 

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