Subpopulations of lymphocytes and monocytes in blood of patients with atrial fibrillation or atrial flutter associated with hypertension

Home/2022, Vol. 10, No. 2/Subpopulations of lymphocytes and monocytes in blood of patients with atrial fibrillation or atrial flutter associated with hypertension

Cell and Organ Transplantology. 2022; 10(2):in press.
DOI: 10.22494/cot.v10i2.141

Subpopulations of lymphocytes and monocytes in blood of patients with atrial fibrillation or atrial flutter associated with hypertension

Talaieva T., Sychov O., Marchenko O., Tretyak I., Vasylynchuk N., Getman T., Romanova O., Stasyshena O., Vavilova L.

  • M. D. Strazhesko National Scientific Center of Cardiology, Clinical and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine 

Abstract

Atrial fibrillation (AF) remains one of the most common arrhythmias, second only to supraventricular extrasystoles, but the universal cause of its occurrence is still unknown. The inflammatory theory of arrhythmogenesis attracts the attention of researchers around the world.
The purpose of the study is to compare the subpopulations of lymphocytes and monocytes in blood of patients with paroxysmal and persistent forms of atrial fibrillation or atrial flutter (AFL) that associated with arterial hypertension.
Materials and methods. The study involved 103 patients with atrial fibrillation and flutter that occurred secondary to hypertension. Depending on the form of arrhythmia, they were divided into three main groups: group I – with paroxysmal form of atrial fibrillation, group II – with a persistent form of atrial fibrillation, group III – with a persistent form of atrial flutter. The control groups included patients with hypertension, but without these arrhythmias and healthy individuals who entered groups IV and V, respectively. The lymphocytes and monocytes subpopulation was assessed by flow cytometry in peripheral blood.
Results and discussion. Analyzing the lymphocyte subpopulations in peripheral blood of patients with atrial fibrillation and flutter (groups I, II and III), it was found that the number of cells with cytotoxic activity (NK and NKT) in both absolute count and percentage values was significantly higher than in healthy individuals. A statistically significant decrease of T-regulatory cells number was found in patients with arrhythmias compared to control groups (p ≤ 0.05). In patients with AF and AFL associated with hypertension, compared to patients with hypertension without these rhythm disturbances or healthy individuals, there is an increased number of classical and intermediate monocytes subpopulations.
Conclusions. In patients with atrial fibrillation and atrial flutter that occurred as a result of hypertension, compared to patients without arrhythmias or healthy people, there is an increased content of pro-inflammatory subpopulations of blood monocytes, T-cytotoxic cells and a decrease in the content of T-regulatory cells.

Key words: atrial fibrillation; atrial flutter; arterial hypertension; lymphocytes; monocytes

 

1. Kornej J, Börschel CS, Benjamin EJ, Schnabel RB. Epidemiology of Atrial Fibrillation in the 21st Century. Circulation Research. 2020; 127:4-20. https://doi.org/10.1161/circresaha.120.316340
https://doi.org/10.1161/CIRCRESAHA.120.316340
PMid:32716709 PMCid:PMC7577553
2. Gerald V. Naccarelli, Helen Varker, Jay Lin, Kathy L.Schulman. Increasing prevalence of Atrial Fibrillation and Flutter in the United States. Am J Cardiol. 2009; 104(11):1534-1539. https://doi.org/https://doi.org/10.1016/j.amjcard.2009.07.022
https://doi.org/10.1016/j.amjcard.2009.07.022
PMid:19932788
3. Staerk L, Wang B, Preis SR, Larson MG, Lubitz SA, Ellinor PT, et al. Lifetime risk of atrial fibrillation according to optimal, borderline, or elevated levels of risk factors: cohort study based on longitudinal data from the Framingham Heart Study. BMJ. 2018; 361:k1453. https://doi.org/10.1136/bmj.k1453
https://doi.org/10.1136/bmj.k1453
PMid:29699974 PMCid:PMC5917175
4. Tsukamoto M, Seta N, Yoshimoto K. CD14 bright CD16+ intermediate monocytes are induced by interleukin-10 and positively correlate with disease activity in rheumatoid arthritis. Arthritis Res Ther. 2017; 19(1):28. https://doi.org/10.1186/s13075-016-1216-6
https://doi.org/10.1186/s13075-016-1216-6
PMid:28183329 PMCid:PMC5301443
5. Verdecchia P, Angeli F, Reboldi G. Hypertension and Atrial Fibrillation Doubts and Certainties From Basic and Clinical Studies. Circulation Research. 2018; 122(2):352-368. https://doi.org/10.1161/circresaha.117.311402
https://doi.org/10.1161/CIRCRESAHA.117.311402
PMid:29348255
6. Kallistratos M, Poulimenos LE, Manolis AJ. Atrial fibrillation and arterial hypertension. Pharmacol Res. 2018; 128:322-326. https://doi.org/10.1016/j.phrs.2017.10.007
https://doi.org/10.1016/j.phrs.2017.10.007
PMid:29055746
7. Boos CJ, Anderson RA, Lip GYH. Is atrial fibrillation an inflammatory disorder? Eur Heart J. 2006; 27(2):136-149. https://doi.org/10.1093/eurheartj/ehi645
https://doi.org/10.1093/eurheartj/ehi645
PMid:16278230
8. HuY-F, Chen YJ, Lin YJ, Chen SA. Inflammation and the pathogenesis of atrial fibrillation. Nat Rev Cardiol. 2015; 12(4), 230-243. https://doi.org/10.1038/nrcardio.2015.2
https://doi.org/10.1038/nrcardio.2015.2
PMid:25622848
9. Ziegler-Heitbrock L, Hofer TP. Toward a refined definition of monocyte subsets. Front Immunol. 2013; (4):23. https://doi.org/10.3389/fimmu.2013.00023
https://doi.org/10.3389/fimmu.2013.00023
PMid:23382732 PMCid:PMC3562996
10. Prabhu SD. It takes two to tango: monocyte and macrophage duality in the infarcted heart. Circ Res. 2014; 114:1558-1560. https://doi.org/10.1161/CIRCRESAHA.114.303933
https://doi.org/10.1161/CIRCRESAHA.114.303933
PMid:24812348 PMCid:PMC4039188
11. Sprangers S, Vries T, Everts V. Monocyte Heterogeneity: Consequences for Monocyte-Derived Immune Cells. J Immunol Res. 2016. https://doi.org/10.1155/2016/1475435
https://doi.org/10.1155/2016/1475435
PMid:27478854 PMCid:PMC4958468
12. Nahrendorf M, Pittet MJ, Swirski FK. Monocytes: protagonists of infarct inflammation and repair after myocardial infarction. Circulation. 2010; 121:2437-2445. https://doi.org/10.1161/CIRCULATIONAHA.109.916346
https://doi.org/10.1161/CIRCULATIONAHA.109.916346
PMid:20530020 PMCid:PMC2892474
13. Suzuki A, Fukuzawa K, Yamashita T, Yoshida A, Sasaki N, Emoto T, et al. Circulating intermediate CD14++CD16+ monocytes are increased in patients with atrial fibrillation and reflect the functional remodelling of the left atrium. EP Europace. 2017. 19(1):40-47. https://doi.org/10.1093/europace/euv422
https://doi.org/10.1093/europace/euv422
PMid:26826137
14. Ghattas A, Griffiths H R, Devitt A, Lip GYH, Shantsila E. Monocytes in Coronary Artery Disease and Atherosclerosis: Where Are We Now? J Am Coll Cardiol. 2013; 62:1541-1551. https://doi.org/10.1016/j.jacc.2013.07.043
https://doi.org/10.1016/j.jacc.2013.07.043
PMid:23973684
15. Zharinov OY, Levchuk NP, Ikorkin MR, Sychev OS. Prediction of prolonged sinus rhythm retention after cardioversion in patients with non-valvular persistent atrial fibrillation. Lviv Clinical Bulletin. 2014; 4(8): 2014.
https://doi.org/10.25040/lkv2014.04.008
16. Joffe HV, Adler GK. Effect of aldosterone and mineralocorticoid receptor blockade on vascular inflammation. Heart Fail Rev. 2005; 10:31-7. https://doi.org/10.1007/s10741-005-2346-0
https://doi.org/10.1007/s10741-005-2346-0
PMid:15947889
17. Zhang J, Yang L, Ding Y. Efects of irbesartan on phenotypic alterations in monocytes and the inflammatory status of hypertensive patients with left ventricular hypertrophy. BMC Cardiovascular Disorders. 2021; 21:194. https://doi.org/10.1186/s12872-021-02004-78
https://doi.org/10.1186/s12872-021-02004-7
PMid:33879070 PMCid:PMC8056615
18. Kumar P, Saini S, Khan S, Surendra Lele S, Prabhakar BS. Restoring self-tolerance in autoimmune diseases by enhancing regulatory T-cells.Cell Immunol. 2019; 339:41-49. https://doi.org/10.1016/j.cellimm.2018.09.008
https://doi.org/10.1016/j.cellimm.2018.09.008
PMid:30482489 PMCid:PMC6440877
19. Bigdelou B, Reza S M, Najafikhoshnoo S, et al. COVID-19 and Preexisting Comorbidities: Risks, Synergies, and Clinical Outcomes. Front Immunol. 2022; 13:890517. https://doi.org/10.3389/fimmu.2022.890517
https://doi.org/10.3389/fimmu.2022.890517
PMid:35711466 PMCid:PMC9196863
20. Shahid F, Lip GYH, Shantsila E. Role of Monocytes in Heart Failure and Atrial Fibrillation. JAHA. 2018; 7(3):e007849. https://doi.org/10.1161/jaha.117.007849
https://doi.org/10.1161/JAHA.117.007849
PMid:29419389 PMCid:PMC5850261
21. Tapp LD, Shantsila E, Wrigley BJ, Pamukcu B, Lip GYH. The CD14++CD16+ monocyte subset and monocyte-platelet interactions in patients with ST-elevation myocardial infarction. JTH. 2012; 10(7). https://doi.org/10.1111/j.1538-7836.2011.04603.x
https://doi.org/10.1111/j.1538-7836.2011.04603.x
PMid:22212813
22. Hindricks G, Potpara T, Dagres N, Arbelo E, Bax J, Blomström-Lundqvist C, et al. ESC Guidelines for the diagnosis and management of atrial fibrillation developed in collaboration with the European Association for Cardio-Thoracic Surgery (EACTS): The Task Force for the diagnosis and management of atrial fibrillation of the European Society of Cardiology (ESC) Developed with the special contribution of the European Heart Rhythm Association (EHRA) of the ESC. 2020. Eur Heart J. 2021; 42(5):373-498. https://doi.org/10.1093/eurheartj/ehaa612
https://doi.org/10.1093/eurheartj/ehaa612
PMid:32860505
23. Herrera-Zelada N, Zuñiga-Cuevas U, Ramirez-Reyes A, Lavandero JA. Riquelme S. Targeting the Endothelium to Achieve Cardioprotection. Front Pharmacol. 2021; 12:1-17. https://doi.org/10.3389/fphar.2021.636134
https://doi.org/10.3389/fphar.2021.636134
PMid:33603675 PMCid:PMC7884828
24. Theofilis P, Sagris M, Oikonomou E, Antonopoulos AS, Siasos G, Tsioufis C, et al. Inflammatory Mechanisms Contributing to Endothelial Dysfunction. Biomedicines. 2021; https://doi.org/10.3390/biomedicines9070781
https://doi.org/10.3390/biomedicines9070781
PMid:34356845 PMCid:PMC8301477

TalaievaT, Sychov O, Marchenko O, Tretyak I, Vasylynchuk N, Getman T, Romanova O, Stasyshena O, Vavilova L. Subpopulations of lymphocytes and monocytes in blood of patients with atrial fibrillation or atrial flutter associated with hypertension. Cell Organ Transpl. 2022; 10(2):in press. doi:10.22494/cot.v10i2.141

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